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rdf:type |
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lifeskim:mentions |
umls-concept:C0001271,
umls-concept:C0015744,
umls-concept:C0017019,
umls-concept:C0017337,
umls-concept:C0040669,
umls-concept:C0205227,
umls-concept:C0220905,
umls-concept:C0596995,
umls-concept:C0871261,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911691,
umls-concept:C2911692
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pubmed:issue |
4
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pubmed:dateCreated |
1992-6-11
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pubmed:abstractText |
We have examined the role of feedback-regulation in the expression of the nonmuscle actin genes. C2 mouse myoblasts were transfected with the human beta- and gamma-actin genes. In gamma-actin transfectants we found that the total actin mRNA and protein pools remained unchanged. Increasing levels of human gamma-actin expression resulted in a progressive down-regulation of mouse beta- and gamma-actin mRNAs. Transfection of the beta-actin gene resulted in an increase in the total actin mRNA and protein pools and induced an increase in the levels of mouse beta-actin mRNA. In contrast, transfection of a beta-actin gene carrying a single-point mutation (beta sm) produced a feedback-regulatory response similar to that of the gamma-actin gene. Expression of a beta-actin gene encoding an unstable actin protein had no impact on the endogenous mouse actin genes. This suggests that the nature of the encoded actin protein determines the feedback-regulatory response of the mouse genes. The role of the actin cytoskeleton in mediating this feedback-regulation was evaluated by disruption of the actin network with Cytochalasin D. We found that treatment with Cytochalasin D abolished the down-regulation of mouse gamma-actin in both the gamma- and beta sm-actin transfectants. In contrast, a similar level of increase was observed for the mouse beta-actin mRNA in both control and transfected cells. These experiments suggest that the down-regulation of mouse gamma-actin mRNA is dependent on the organization of the actin cytoskeleton. In addition, the mechanism responsible for the down-regulation of beta-actin may be distinct from that governing gamma-actin. We conclude that actin feedback-regulation provides a biochemical assay for differences between the two nonmuscle actin genes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-1577857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-1690676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-1993736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-2078553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-2120242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-2127699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-2204811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-2269338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-2347376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-2420586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-2444342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-2460261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-2479547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-2837653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-2877095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-2996000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-3010322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-3380097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-3405308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-3431550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-3614198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-3614199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-3737401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-3837182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-455467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-5963888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-6835208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-6893015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1577858-7199055
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
117
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
787-97
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:1577858-Actin Cytoskeleton,
pubmed-meshheading:1577858-Actins,
pubmed-meshheading:1577858-Animals,
pubmed-meshheading:1577858-Cells, Cultured,
pubmed-meshheading:1577858-Gene Expression Regulation,
pubmed-meshheading:1577858-Humans,
pubmed-meshheading:1577858-Mice,
pubmed-meshheading:1577858-Muscles,
pubmed-meshheading:1577858-RNA, Messenger,
pubmed-meshheading:1577858-Transfection
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pubmed:year |
1992
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pubmed:articleTitle |
Transfection of nonmuscle beta- and gamma-actin genes into myoblasts elicits different feedback regulatory responses from endogenous actin genes.
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pubmed:affiliation |
Cell Biology Unit, Children's Medical Research Foundation, Camperdown, N.S.W., Australia.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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