Linked Life Data

15745961

Source:http://linkedlifedata.com/resource/pubmed/id/15745961

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2005-3-4
pubmed:abstractText
Shaker, a voltage-dependent K+ channel, is enriched in the mushroom bodies (MBs), the locus of olfactory learning in Drosophila. Mutations in the shaker locus are known to alter excitability, neurotransmitter release, synaptic plasticity, and olfactory learning. However, a direct link of Shaker channels to MB intrinsic neuron (MBN) physiology has not been documented. We found that transcripts for shab, shaw, shaker, and shal, among which only Shaker and Shal have been reported to code for A-type currents, are present in the MBs. The electrophysiological data showed that the absence of functional Shaker channels modifies the distribution of half-inactivation voltages (V(i1/2)) in the MBNs, indicating a segregation of Shaker channels to only a subset (approximately 28%) of their somata. In harmony with this notion, we found that approximately one-fifth of MBNs lacking functional Shaker channels displayed dramatically slowed-down outward current inactivation times and reduced peak-current amplitudes. Furthermore, whereas all MBNs were sensitive to 4-aminopyridine, a nonspecific A-type current blocker, a subset of neurons (approximately 24%) displayed little sensitivity to a Shal-specific toxin. This subset of neurons displaying toxin-insensitive outward currents had more depolarized V(i1/2) values attributable to Shaker channels. Our findings provide the first direct evidence that altered Shaker channel function disrupts MBN physiology in Drosophila. To our surprise, the experimental data also indicate that Shaker channels segregate to a minor fraction of MB neuronal somata (20-30%), and that Shal channels contribute the somatic A-type current in the majority of MBNs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
2
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2348-58
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15745961-4-Aminopyridine, pubmed-meshheading:15745961-Animals, pubmed-meshheading:15745961-Animals, Genetically Modified, pubmed-meshheading:15745961-Blotting, Northern, pubmed-meshheading:15745961-Chi-Square Distribution, pubmed-meshheading:15745961-Dose-Response Relationship, Radiation, pubmed-meshheading:15745961-Drosophila, pubmed-meshheading:15745961-Drosophila Proteins, pubmed-meshheading:15745961-Green Fluorescent Proteins, pubmed-meshheading:15745961-Larva, pubmed-meshheading:15745961-Membrane Potentials, pubmed-meshheading:15745961-Mushroom Bodies, pubmed-meshheading:15745961-Mutagenesis, pubmed-meshheading:15745961-Mutation, pubmed-meshheading:15745961-Neurons, pubmed-meshheading:15745961-Patch-Clamp Techniques, pubmed-meshheading:15745961-Potassium, pubmed-meshheading:15745961-Potassium Channel Blockers, pubmed-meshheading:15745961-RNA, Messenger, pubmed-meshheading:15745961-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15745961-Shaker Superfamily of Potassium Channels, pubmed-meshheading:15745961-Shal Potassium Channels
pubmed:year
2005
pubmed:articleTitle
Shal and shaker differential contribution to the K+ currents in the Drosophila mushroom body neurons.
pubmed:affiliation
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62210, México, and Centro de Estudios Científicos, Casilla 1469, Valdivia, Chile.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't