Linked Life Data

15714578

Source:http://linkedlifedata.com/resource/pubmed/id/15714578

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4-5
pubmed:dateCreated
2005-2-24
pubmed:abstractText
Enantioseparation of chiral aryl allenic acids by micellar electrokinetic chromatography (MEKC) with cyclodextrins (CDs) as chiral selectors was described. The screen of chiral selectors (beta-CD, gamma-CD, and hydroxypropyl (HP)-gamma-CD) showed that the enantioseparation was not only dependent on the type of CD but also the presence of 2-propanol in the buffer. In order to optimize the operational parameters, the effect of the concentration of CDs, sodium dodecyl sulfate (SDS), and 2-propanol, as well as the buffer ionic strength and pH on enantioseparation were studied. It was proved that the concentration of CDs, 2-propanol, and the buffer ionic strength were the critical parameters. Under optimal conditions, baseline separations of all seven allenic acid enantiomers were achieved. Furthermore, the method validation in terms of repeatability, linearity, limit of detection (LOD), and limit of quantitation (LOQ) were performed. Using the present method, the optical purity of a nonracemic sample with the enantiomeric excess (e.e.%) value of 99.65% was determined.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0173-0835
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1001-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Enantioseparation of chiral allenic acids by micellar electrokinetic chromatography with cyclodextrins as chiral selector.
pubmed:affiliation
State Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Graduate School of Chinese Academy of Sciences, Shanghai, China.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Validation Studies