Source:http://linkedlifedata.com/resource/pubmed/id/15714493
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2005-2-22
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pubmed:abstractText |
Human papillomaviruses (HPVs) are etiological agents of cervical cancer. In order to assess the epidemiological incidence and frequency of different HPV types, we applied a polymerase chain reaction (PCR)-direct sequencing approach based on the use of MY09/MY11 primers as compared to Hybrid Capture assay. Cervical samples were taken from 1,500 women, both with normal and abnormal cytological smears, and we found an incidence of 6.6% of HPV infection in Brescia. Overall, 97 samples tested HPV-positive, yielding 18 HPV types. The four most frequent HPV types were: HPV 16, -31, -6, and -58. This approach could be used in ordinary laboratory settings for quick and reliable typing of known and novel HPVs from clinical specimens and it could also be applied to anti-cancer vaccine development.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0146-6615
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pubmed:author | |
pubmed:copyrightInfo |
(c) 2005 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
588-92
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15714493-Adolescent,
pubmed-meshheading:15714493-Adult,
pubmed-meshheading:15714493-Carcinoma in Situ,
pubmed-meshheading:15714493-Cervical Intraepithelial Neoplasia,
pubmed-meshheading:15714493-Cervix Uteri,
pubmed-meshheading:15714493-Female,
pubmed-meshheading:15714493-Genotype,
pubmed-meshheading:15714493-Humans,
pubmed-meshheading:15714493-Italy,
pubmed-meshheading:15714493-Middle Aged,
pubmed-meshheading:15714493-Papillomaviridae,
pubmed-meshheading:15714493-Papillomavirus Infections,
pubmed-meshheading:15714493-Sequence Analysis, DNA,
pubmed-meshheading:15714493-Uterine Cervical Neoplasms
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pubmed:year |
2005
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pubmed:articleTitle |
Detection and genotyping of human papillomavirus in cervical samples from Italian patients.
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pubmed:affiliation |
Institute of Microbiology and Virology, Spedali Civili-University of Brescia, 1 Piazzale Spedali Civili, 25123 Brescia, Italy. defrance@med.unibs.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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