Source:http://linkedlifedata.com/resource/pubmed/id/15680955
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
2005-1-31
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pubmed:abstractText |
Gastrin-releasing peptide (GRP) is a mammalian bombesin (BN)-like peptide that binds with high affinity to the GRP receptor (GRP-R). Previous behavioral studies using mice and rats showed that the GRP/GRP-R system mediates learning and memory by modulating neurotransmitter release in the local GABAergic network of the amygdala and the nucleus tractus solitarius (NTS). To date, the precise distribution of GRP-R in the brain has not been elucidated. We used a synthetic peptide derived from mouse GRP-R to generate affinity-purified antibodies to GRP-R and used immunohistochemistry to determine the distribution of GRP-R in the mouse brain. The specificity of anti-GRP-R antibody was confirmed in vitro using COS-7 cells transiently expressing GRP-R and in vivo using GRP-R-deficient and wild-type mouse brain sections. GRP-R immunoreactivity was widely distributed in the isocortex, hippocampal formation, piriform cortex, amygdala, hypothalamus, and brain stem. In particular, GRP-R immunoreactivity was observed in the lateral (LA), central, and basolateral amygdaloid (BLA) nuclei and NTS, which are important regions for memory performance. Double-labeling immunohistochemistry demonstrated that subpopulations of GRP-R are present in GABAergic neurons in the amygdala. Consequently, GRP-R immunoreactivity was observed in the GABAergic neurons of the limbic region. These anatomical results provide support for the idea that the GRP/GRP-R system mediates memory performance by modulating neurotransmitter release in the local GABAergic network.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Glial Fibrillary Acidic Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamate Decarboxylase,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mtap2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphopyruvate Hydratase,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Bombesin,
http://linkedlifedata.com/resource/pubmed/chemical/glutamate decarboxylase 1
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-8993
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
1032
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
162-70
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pubmed:dateRevised |
2007-10-24
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pubmed:meshHeading |
pubmed-meshheading:15680955-Animals,
pubmed-meshheading:15680955-Antibodies,
pubmed-meshheading:15680955-Antibody Specificity,
pubmed-meshheading:15680955-Brain,
pubmed-meshheading:15680955-COS Cells,
pubmed-meshheading:15680955-Cercopithecus aethiops,
pubmed-meshheading:15680955-Glial Fibrillary Acidic Protein,
pubmed-meshheading:15680955-Glutamate Decarboxylase,
pubmed-meshheading:15680955-Immunohistochemistry,
pubmed-meshheading:15680955-Isoenzymes,
pubmed-meshheading:15680955-Male,
pubmed-meshheading:15680955-Mice,
pubmed-meshheading:15680955-Mice, Knockout,
pubmed-meshheading:15680955-Microtubule-Associated Proteins,
pubmed-meshheading:15680955-Phosphopyruvate Hydratase,
pubmed-meshheading:15680955-Receptors, Bombesin,
pubmed-meshheading:15680955-Transfection
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pubmed:year |
2005
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pubmed:articleTitle |
Immunohistochemical localization of gastrin-releasing peptide receptor in the mouse brain.
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pubmed:affiliation |
Department of Cell Biology, School of Human Sciences, Waseda University, 2-579-15, Mikajima, Tokorozawa, Saitama 359-1192, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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