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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2005-1-6
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pubmed:abstractText |
Twenty clinical samples (18 cerebrospinal fluid samples and 2 articular fluid samples) were sent to 11 meningococcus reference centers located in 11 different countries. Ten of these laboratories are participating in the EU-MenNet program (a European Union-funded program) and are members of the European Monitoring Group on Meningococci. The remaining laboratory was located in Burkina Faso. Neisseria meningitidis was sought by detecting several meningococcus-specific genes (crgA, ctrA, 16S rRNA, and porA). The PCR-based nonculture method for the detection of N. meningitidis gave similar results between participants with a mean sensitivity and specificity of 89.7 and 92.7%, respectively. Most of the laboratories also performed genogrouping assays (siaD and mynB/sacC). The performance of genogrouping was more variable between laboratories, with a mean sensitivity of 72.7%. Genogroup B gave the best correlation between participants, as all laboratories routinely perform this PCR. The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these PCRs.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-10024433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-10325326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-10555280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-10576127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-10655397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-10790092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-10795366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-10799809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-11283086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-12002532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-12454147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-12682182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-12701687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-12748270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-1371768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-14556993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-14606610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-14718100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-14729931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-15071043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-1675709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-8224787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-8735116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-8792481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-9129587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-9224883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-9503614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-9515923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-9627955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-9665992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-9692604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15634963-9718842
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0095-1137
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pubmed:author |
pubmed-author:AlonsoJean-MichelJM,
pubmed-author:CafferkeyMaryM,
pubmed-author:CaugantDominique ADA,
pubmed-author:ClarkeStuart CSC,
pubmed-author:DiggleMathew AMA,
pubmed-author:FoxAndrewA,
pubmed-author:FroschMatthiasM,
pubmed-author:GrayStephen JSJ,
pubmed-author:GuiverMalcolmM,
pubmed-author:HeubergerSigridS,
pubmed-author:KalmusovaJitkaJ,
pubmed-author:KesanopoulosKonstantinosK,
pubmed-author:KlemAnne-MarieAM,
pubmed-author:KrizPaulaP,
pubmed-author:MöllingPaulaP,
pubmed-author:MarshJohnJ,
pubmed-author:MurphyKarenK,
pubmed-author:OlcénPerP,
pubmed-author:SanouOumarO,
pubmed-author:TahaMuhamed-KheirMK,
pubmed-author:TzanakakiGeorginaG,
pubmed-author:VogelUlrichU
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pubmed:issnType |
Print
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
144-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15634963-Adolescent,
pubmed-meshheading:15634963-Adult,
pubmed-meshheading:15634963-Burkina Faso,
pubmed-meshheading:15634963-Child,
pubmed-meshheading:15634963-Child, Preschool,
pubmed-meshheading:15634963-DNA, Bacterial,
pubmed-meshheading:15634963-European Union,
pubmed-meshheading:15634963-Female,
pubmed-meshheading:15634963-Genotype,
pubmed-meshheading:15634963-Humans,
pubmed-meshheading:15634963-Infant,
pubmed-meshheading:15634963-Laboratories,
pubmed-meshheading:15634963-Male,
pubmed-meshheading:15634963-Meningitis, Meningococcal,
pubmed-meshheading:15634963-Meningococcal Infections,
pubmed-meshheading:15634963-Neisseria meningitidis,
pubmed-meshheading:15634963-Polymerase Chain Reaction,
pubmed-meshheading:15634963-Sensitivity and Specificity
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pubmed:year |
2005
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pubmed:articleTitle |
Interlaboratory comparison of PCR-based identification and genogrouping of Neisseria meningitidis.
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pubmed:affiliation |
Neisseria Unit and the French National Reference Center for Meningococci, Institut Pasteur, Paris, France. mktaha@pasteur.fr
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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