|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2004-12-30
|
|
pubmed:abstractText |
It is important to elucidate whether the leptin receptor, especially the long signal-transducing form of the leptin receptor (OB-Rb) is expressed in human osteoblasts. We detected the expression of human OB-Rb in cultured commercially available human osteoblasts (NHOst cells) using real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR). After confirming the expression of OB-Rb, we investigated the effect of leptin on NHOst cells. Leptin enhanced cell proliferation of the cells shown by the MTT assay. Furthermore, leptin changed the copy numbers of Bax and Bcl-2 mRNAs in the cultured cells as shown by real-time quantitative RT-PCR, although the effect was not consistent. Leptin did not change the production of osteocalcin and osteopontin by the cells. Leptin did not change the expression of OB-Rb mRNA in the cells. In conclusion, OB-Rb mRNA is expressed in cultured commercially available human osteoblasts. Leptin may have some effects on bone metabolism by directly modulating cell proliferation and apoptosis of osteoblasts in humans.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/BAX protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Leptin,
http://linkedlifedata.com/resource/pubmed/chemical/Osteocalcin,
http://linkedlifedata.com/resource/pubmed/chemical/Osteopontin,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Leptin,
http://linkedlifedata.com/resource/pubmed/chemical/SPP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein,
http://linkedlifedata.com/resource/pubmed/chemical/leptin receptor, human
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0951-3590
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
19
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
97-104
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:15624271-Apoptosis,
pubmed-meshheading:15624271-Cell Division,
pubmed-meshheading:15624271-Cells, Cultured,
pubmed-meshheading:15624271-Genes, bcl-2,
pubmed-meshheading:15624271-Humans,
pubmed-meshheading:15624271-Leptin,
pubmed-meshheading:15624271-Osteoblasts,
pubmed-meshheading:15624271-Osteocalcin,
pubmed-meshheading:15624271-Osteopontin,
pubmed-meshheading:15624271-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:15624271-RNA, Messenger,
pubmed-meshheading:15624271-Receptors, Cell Surface,
pubmed-meshheading:15624271-Receptors, Leptin,
pubmed-meshheading:15624271-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15624271-Sialoglycoproteins,
pubmed-meshheading:15624271-bcl-2-Associated X Protein
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
The leptin receptor in human osteoblasts and the direct effect of leptin on bone metabolism.
|
|
pubmed:affiliation |
Department of Obstetrics and Gynecology, Faculty of Medicine, Kagoshima University, Kagoshima, Japan. ichiro@m.kufm.kagoshima-u.ac.jp
|
|
pubmed:publicationType |
Journal Article
|
