Linked Life Data

15588510

Source:http://linkedlifedata.com/resource/pubmed/id/15588510

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2004-12-13
pubmed:abstractText
Several reports have shown that the expression of Sca-1 (Ly-6A/E), the most widely used murine hematopoietic stem cell marker, is restricted to blood vessels in several nonhematopoietic tissues. However, there is no information about which components are expressing Sca-1, and what the role of Sca-1 could be. Because we have previously shown that murine liver endothelial cells from the hepatic sinusoid (LSEC) express some HSC markers (i. e., CD34 and c-kit), we hypothesized that these cells could also express Sca-1. In this work, we show that Sca-1 is constitutively expressed in LSEC, as well as in the liver sinusoid lumen. The expression of Sca-1 in LSEC was confirmed at the mRNA and protein level by reverse transcriptase (RT)-PCR, flow cytometry, and immunofluorescence studies. The expression of Sca-1 was enhanced on the surface of LSEC by tumor necrosis factor (TNF). We examined whether Sca-1 ligation on the surface of LSEC regulates some biological response in these cells. Our results show that ligation of Sca-1 by the anti-Ly-6A/E monoclonal antibody (mAb) D7 stimulated the growth of LSEC and the production of interleukin-6 (IL-6) by these cells. To our knowledge, this is the first report that LSEC express Sca-1, which may constitute additional support to the theory of a common progenitor for the hematopoietic and endothelial cells. Our results show a novel role of Sca-1, indicating that it induces activation of LSEC to proliferate and to produce IL-6. These results suggest that Sca-1 may participate in several clinical conditions such as angiogenesis and inflammation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1547-3287
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
528-35
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15588510-Animals, pubmed-meshheading:15588510-Antibodies, Monoclonal, pubmed-meshheading:15588510-Antigens, Ly, pubmed-meshheading:15588510-Cell Proliferation, pubmed-meshheading:15588510-Cells, Cultured, pubmed-meshheading:15588510-Cloning, Molecular, pubmed-meshheading:15588510-Endothelial Cells, pubmed-meshheading:15588510-Flow Cytometry, pubmed-meshheading:15588510-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:15588510-Hematopoietic Stem Cells, pubmed-meshheading:15588510-Inflammation, pubmed-meshheading:15588510-Interleukin-18, pubmed-meshheading:15588510-Interleukin-6, pubmed-meshheading:15588510-Liver, pubmed-meshheading:15588510-Membrane Proteins, pubmed-meshheading:15588510-Mice, pubmed-meshheading:15588510-Microscopy, Fluorescence, pubmed-meshheading:15588510-Neovascularization, Pathologic, pubmed-meshheading:15588510-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15588510-Time Factors, pubmed-meshheading:15588510-Tumor Necrosis Factor-alpha
pubmed:year
2004
pubmed:articleTitle
Expression of the hematopoietic stem cell antigen Sca-1 (LY-6A/E) in liver sinusoidal endothelial cells: possible function of Sca-1 in endothelial cells.
pubmed:affiliation
Laboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas 1020-A, Venezuela.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't