Linked Life Data

15571953

Source:http://linkedlifedata.com/resource/pubmed/id/15571953

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2004-12-1
pubmed:abstractText
The aim of this study was to investigate the expression of Zonula Occludens (ZO) proteins ZO-1, ZO-2 and ZO-3, and MUPP-1 (multi-PDZ domain protein 1), peripheral/plaque proteins that function in maintaining tight junction integrity and in transducing regulatory signalling events in patients with primary breast cancer. Breast cancer primary tumours (n=114) and background tissues (n=30) were processed for quantitative-polymerase chain reaction (PCR) analysis, Western blotting and immunostaining. Standardised transcript levels of ZO-1 and MUPP-1 were significantly lower in patients with metastatic disease compared with those remaining disease-free (DF) (median follow-up 72.2 months). Immunohistochemistry confirmed these results, with decreased levels in ZO-1 staining. For both ZO-1 and ZO-3, staining was confined to the intercellular regions in normal tissue, whereas in tumour tissues staining was diffuse and cytosolic. Q-PCR revealed a reduction in the levels of ZO-1 and MUPP-1 in patients with disease recurrence. Prognostic indicators of breast cancer were also inversely correlated with ZO-1 expression. We conclude that low levels of tight junction plaque molecules, such as ZO-1 and MUPP-1, in breast cancer are associated with poor patients prognosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0959-8049
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2717-25
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Loss of tight junction plaque molecules in breast cancer tissues is associated with a poor prognosis in patients with breast cancer.
pubmed:affiliation
Metastasis and Angiogenesis Research Group, Department of Surgery, University of Wales College of Medicine, Heath Park, Cardiff, Wales CF14 4XN, UK. martina1@cf.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't