Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2004-11-16
pubmed:abstractText
Both reactive oxygen- and nitrogen-derived reactive species play important roles in physiological and pathophysiological conditions. Flavones, luteolin and luteolin-7-O-glucoside along with a rich plant source of both flavones, namely dandelion (Taraxacum officinale) flower extract were studied for antioxidant activity in different in vitro model systems. In this current study, luteolin and luteolin-7-O-glucoside at concentrations lower than 20 microM, significantly (p < 0.05) suppressed the productions of nitric oxide and prostaglandin E2 (PGE2) in bacterial lipopolysaccharide activated-mouse macrophage RAW264.7 cells without introducing cytotoxicity. The inhibitory effects were further attributed to the suppression of both inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression, and not reduced enzymatic activity. Similar suppression for both inducible enzymes was also found with the presence of dandelion flower extract, specifically, the ethyl acetate fraction of dandelion flower extract which contained 10% luteolin and luteolin-7-O-glucoside.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants, http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2, http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2 Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone, http://linkedlifedata.com/resource/pubmed/chemical/Drugs, Chinese Herbal, http://linkedlifedata.com/resource/pubmed/chemical/Flavones, http://linkedlifedata.com/resource/pubmed/chemical/Glucosides, http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides, http://linkedlifedata.com/resource/pubmed/chemical/Luteolin, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II, http://linkedlifedata.com/resource/pubmed/chemical/Nitrites, http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Oxygen, http://linkedlifedata.com/resource/pubmed/chemical/Plant Extracts, http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases, http://linkedlifedata.com/resource/pubmed/chemical/Reactive Nitrogen Species, http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species, http://linkedlifedata.com/resource/pubmed/chemical/luteolin-7-glucoside
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0300-8177
pubmed:author
pubmed:issnType
Print
pubmed:volume
265
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
107-13
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15543940-Animals, pubmed-meshheading:15543940-Antioxidants, pubmed-meshheading:15543940-Blotting, Western, pubmed-meshheading:15543940-Cell Line, pubmed-meshheading:15543940-Cell Survival, pubmed-meshheading:15543940-Cyclooxygenase 2, pubmed-meshheading:15543940-Cyclooxygenase 2 Inhibitors, pubmed-meshheading:15543940-Cyclooxygenase Inhibitors, pubmed-meshheading:15543940-Dinoprostone, pubmed-meshheading:15543940-Dose-Response Relationship, Drug, pubmed-meshheading:15543940-Drugs, Chinese Herbal, pubmed-meshheading:15543940-Flavones, pubmed-meshheading:15543940-Glucosides, pubmed-meshheading:15543940-Lipopolysaccharides, pubmed-meshheading:15543940-Luteolin, pubmed-meshheading:15543940-Mice, pubmed-meshheading:15543940-Nitric Oxide Synthase, pubmed-meshheading:15543940-Nitric Oxide Synthase Type II, pubmed-meshheading:15543940-Nitrites, pubmed-meshheading:15543940-Oxygen, pubmed-meshheading:15543940-Plant Extracts, pubmed-meshheading:15543940-Plants, pubmed-meshheading:15543940-Prostaglandin-Endoperoxide Synthases, pubmed-meshheading:15543940-Reactive Nitrogen Species, pubmed-meshheading:15543940-Reactive Oxygen Species, pubmed-meshheading:15543940-Taraxacum
pubmed:year
2004
pubmed:articleTitle
Luteolin and luteolin-7-O-glucoside from dandelion flower suppress iNOS and COX-2 in RAW264.7 cells.
pubmed:affiliation
Food, Nutrition and Health, Faculty of Agricultural Sciences, University of British Columbia, Vancouver, BC, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't