Linked Life Data

15509807

Source:http://linkedlifedata.com/resource/pubmed/id/15509807

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2004-10-28
pubmed:abstractText
Transcriptional activity of the TATA-binding protein (TBP) is controlled by a variety of proteins. The BTAF1 protein (formerly known as TAF(II)170/TAF-172 and the human ortholog of Saccharomyces cerevisiae Mot1p) and the NC2 complex composed of NC2alpha (DRAP1) and NC2beta (Dr1) are able to bind to TBP directly and regulate RNA polymerase II transcription both positively and negatively. Here, we present evidence that the NC2alpha subunit interacts with BTAF1. In contrast, the NC2beta subunit is not able to associate with BTAF1 and seems to interfere with the BTAF1-TBP interaction. Addition of NC2alpha or the NC2 complex can stimulate the ability of BTAF1 to interact with TBP. This function is dependent on the presence of ATP in cell extracts but does not involve the ATPase activity of BTAF1 nor phosphorylation of NC2alpha. Together, our results constitute the first evidence of the physical cooperation between BTAF1 and NC2alpha in TBP regulation and provide a framework to understand transcription functions of NC2alpha and NC2beta in vivo.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-10082549, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-10713169, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-10760173, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-10974559, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-11062130, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-11283253, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-11461703, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-11585931, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-11880621, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-11909516, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-11909519, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-12191469, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-12237409, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-12356733, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-12417716, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-12471260, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-12502746, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-12571241, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-12612638, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-12805227, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-12878007, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-1312673, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-1339312, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-1387711, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-14557059, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-14988402, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-15001359, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-1934060, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-1936986, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-7958867, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-7984417, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-8083216, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-8313884, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-8340360, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-8454623, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-8608938, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-8625415, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-8670811, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-8943321, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-8948634, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-8972183, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-8972209, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-9121454, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-9234740, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-9342322, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-9488487, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-9585500, http://linkedlifedata.com/resource/pubmed/commentcorrection/15509807-9671455
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10072-82
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
NC2alpha interacts with BTAF1 and stimulates its ATP-dependent association with TATA-binding protein.
pubmed:affiliation
Department of Physiological Chemistry, Division of Biomedical Genetics, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands.
pubmed:publicationType
Journal Article, In Vitro
More...