| pubmed-article:15501477 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15501477 | lifeskim:mentions | umls-concept:C1704256 | lld:lifeskim |
| pubmed-article:15501477 | lifeskim:mentions | umls-concept:C1523298 | lld:lifeskim |
| pubmed-article:15501477 | lifeskim:mentions | umls-concept:C1440080 | lld:lifeskim |
| pubmed-article:15501477 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:15501477 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:15501477 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:15501477 | pubmed:dateCreated | 2004-10-25 | lld:pubmed |
| pubmed-article:15501477 | pubmed:abstractText | The aim of this study was to investigate whether transforming growth factor-beta1 (TGF-beta1) could induce alveolar epithelial to mesenchymal transition (EMT) in vitro. Alveolar epithelial cells (AECs) from SD rats were isolated by elastase cell dispersion and IgG panning. Expression of alpha-smooth muscle actin (alpha-SMA) was assayed using Western blotting and immunostaining analysis. Morphological changes, the markers of epithelial cell (E-cadherin), and stress fiber by actin reorganization were detected by an indirect immunostaining. The contents of collagen I were determined by spectrophotometry. The levels of endogenous TGF-beta1 were measured with ELISA. Incubation of AECs with TGF-beta1 (0.1 approximately 10 ng/mL) induced abundant expression of alpha-SMA protein, and alpha-SMA expression in AECs reached a plateau when TGF-beta1 was > 3 ng/mL. Furthermore, we found that TGF-beta1 (3 ng/mL) exposure of AECs induced an authentic EMT characterized by abundant expression of alpha-smooth muscle actin, transformation of myofibroblastic morphology, increased formation of stress fiber by actin reorganization, and loss of epithelial marker E-cadherin. Meanwhile, significant increase in the levels of collagen I from 32.0 +/- 6.6 mg/g in control to 98 +/- 10.8 mg/g in TGF-beta1-treated group was found over a 72 h incubation period. Moreover, following stimulated by TGF-beta1 (3 ng/mL), a marked and time-dependent increase in endogenous TGF-beta1 released from AECs was observed. At time points 72 h, TGF-beta1 release mounted to 3451 pg/ml, which was much enough to induce EMT in vitro. These results demonstrated that AECs, under stimulation of TGF-beta1, underwent a conversion process into myofibroblasts in vitro. | lld:pubmed |
| pubmed-article:15501477 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15501477 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15501477 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15501477 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:15501477 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15501477 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:15501477 | pubmed:issn | 0024-3205 | lld:pubmed |
| pubmed-article:15501477 | pubmed:author | pubmed-author:ChenJi-QiangJ... | lld:pubmed |
| pubmed-article:15501477 | pubmed:author | pubmed-author:XieQiang-MinQ... | lld:pubmed |
| pubmed-article:15501477 | pubmed:author | pubmed-author:YaoHong-WeiHW | lld:pubmed |
| pubmed-article:15501477 | pubmed:author | pubmed-author:TangHui-FangH... | lld:pubmed |
| pubmed-article:15501477 | pubmed:author | pubmed-author:DengYang-MeiY... | lld:pubmed |
| pubmed-article:15501477 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15501477 | pubmed:day | 19 | lld:pubmed |
| pubmed-article:15501477 | pubmed:volume | 76 | lld:pubmed |
| pubmed-article:15501477 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15501477 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15501477 | pubmed:pagination | 29-37 | lld:pubmed |
| pubmed-article:15501477 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:15501477 | pubmed:meshHeading | pubmed-meshheading:15501477... | lld:pubmed |
| pubmed-article:15501477 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15501477 | pubmed:articleTitle | TGF-beta1 induces alveolar epithelial to mesenchymal transition in vitro. | lld:pubmed |
| pubmed-article:15501477 | pubmed:affiliation | Zhejiang Respiratory Drugs Research Laboratory of State Food Drugs Administration of China, School of Medicine, Zhejiang University, Hangzhou 310031, China. yhgwei@hotmail.com | lld:pubmed |
| pubmed-article:15501477 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15501477 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:15501477 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:15501477 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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