Source:http://linkedlifedata.com/resource/pubmed/id/15501477
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2004-10-25
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| pubmed:abstractText |
The aim of this study was to investigate whether transforming growth factor-beta1 (TGF-beta1) could induce alveolar epithelial to mesenchymal transition (EMT) in vitro. Alveolar epithelial cells (AECs) from SD rats were isolated by elastase cell dispersion and IgG panning. Expression of alpha-smooth muscle actin (alpha-SMA) was assayed using Western blotting and immunostaining analysis. Morphological changes, the markers of epithelial cell (E-cadherin), and stress fiber by actin reorganization were detected by an indirect immunostaining. The contents of collagen I were determined by spectrophotometry. The levels of endogenous TGF-beta1 were measured with ELISA. Incubation of AECs with TGF-beta1 (0.1 approximately 10 ng/mL) induced abundant expression of alpha-SMA protein, and alpha-SMA expression in AECs reached a plateau when TGF-beta1 was > 3 ng/mL. Furthermore, we found that TGF-beta1 (3 ng/mL) exposure of AECs induced an authentic EMT characterized by abundant expression of alpha-smooth muscle actin, transformation of myofibroblastic morphology, increased formation of stress fiber by actin reorganization, and loss of epithelial marker E-cadherin. Meanwhile, significant increase in the levels of collagen I from 32.0 +/- 6.6 mg/g in control to 98 +/- 10.8 mg/g in TGF-beta1-treated group was found over a 72 h incubation period. Moreover, following stimulated by TGF-beta1 (3 ng/mL), a marked and time-dependent increase in endogenous TGF-beta1 released from AECs was observed. At time points 72 h, TGF-beta1 release mounted to 3451 pg/ml, which was much enough to induce EMT in vitro. These results demonstrated that AECs, under stimulation of TGF-beta1, underwent a conversion process into myofibroblasts in vitro.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0024-3205
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
19
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| pubmed:volume |
76
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
29-37
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15501477-Actins,
pubmed-meshheading:15501477-Animals,
pubmed-meshheading:15501477-Cadherins,
pubmed-meshheading:15501477-Cells, Cultured,
pubmed-meshheading:15501477-Collagen Type I,
pubmed-meshheading:15501477-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15501477-Epithelium,
pubmed-meshheading:15501477-Immunoblotting,
pubmed-meshheading:15501477-Immunohistochemistry,
pubmed-meshheading:15501477-Male,
pubmed-meshheading:15501477-Mesoderm,
pubmed-meshheading:15501477-Muscle, Smooth,
pubmed-meshheading:15501477-Pulmonary Alveoli,
pubmed-meshheading:15501477-Pulmonary Fibrosis,
pubmed-meshheading:15501477-Rats,
pubmed-meshheading:15501477-Rats, Sprague-Dawley,
pubmed-meshheading:15501477-Spectrophotometry,
pubmed-meshheading:15501477-Time Factors,
pubmed-meshheading:15501477-Transforming Growth Factor beta
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
TGF-beta1 induces alveolar epithelial to mesenchymal transition in vitro.
|
| pubmed:affiliation |
Zhejiang Respiratory Drugs Research Laboratory of State Food Drugs Administration of China, School of Medicine, Zhejiang University, Hangzhou 310031, China. yhgwei@hotmail.com
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| pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't
|