Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2004-11-10
pubmed:abstractText
Expression of the castration-induced clusterin protein is incompatible with the survival of human prostate cancer cells in tissues and in cell culture. To investigate the fate of human prostate epithelial cells, when engineered to maintain expression of clusterin protein, we have used an IRES-hyg vector and hygromycin selection. PC-3 prostate tumour cells were substantially more sensitive to clusterin expression than nonmalignant PNT1a cells, showing multiple phenotypic changes including cell cycle arrest and increased apoptosis. The results strengthen the hypothesis that clusterin expression is proapoptotic. Expression of exogenous clusterin in both cell types resulted in its relocation from the cytoplasm and a nuclear accumulation of the protein, as was also seen in the same cells when apoptosis was induced by etoposide treatment. To survive clusterin expression, the PC-3 tumour cells developed apoptosis-inhibitory properties. This could have significance for the resistance of prostate cancers to chemo/radiotherapy, where clusterin overexpression is observed.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-10218496, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-10646846, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-10646870, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-10993660, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-11105915, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-11168651, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-11231633, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-11358798, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-11420705, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-11641613, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-11741605, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-11796313, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-11813210, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-11857034, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-11904161, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-12082621, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-12409141, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-12551933, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-12679903, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-14618611, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-14996747, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-2920020, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-6863260, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-7115849, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-7648419, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-7654321, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-8034050, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-8770413, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-8910583, http://linkedlifedata.com/resource/pubmed/commentcorrection/15494717-9426051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0007-0920
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1842-50
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Clusterin overexpression in both malignant and nonmalignant prostate epithelial cells induces cell cycle arrest and apoptosis.
pubmed:affiliation
Dipartimento di Scienze Biomediche, Universita' di Modena e Reggio Emilia, Via GCampi, Modena 287-41100, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't