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rdf:type |
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lifeskim:mentions |
umls-concept:C0004112,
umls-concept:C0006104,
umls-concept:C0017262,
umls-concept:C0022116,
umls-concept:C0026809,
umls-concept:C0063695,
umls-concept:C0205234,
umls-concept:C0205374,
umls-concept:C0205421,
umls-concept:C0255808,
umls-concept:C1171362,
umls-concept:C1515670
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pubmed:issue |
1
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pubmed:dateCreated |
2004-10-18
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pubmed:abstractText |
Intercellular adhesion molecule-1 (ICAM-1) is expressed after brain ischemia and is participated in the induction of neuronal cell death. Recently, we have reported that ICAM-1 is localized in astrocytes in the chronic phase of ischemia. However, the regulation of astroglial ICAM-1 after brain ischemia is not elucidated in detail. Therefore, we examined the gene and protein expression of TNFR1 after transient middle cerebral artery occlusion (tMCAO) by using real time-PCR and immunohistochemistry. Moreover, we determined the relationship of TNFR1 and ICAM-1 in the astrocyte in chronic phase of ischemia. Increased expression of TNFR1 mRNA in the ipsilateral cortex was noted slightly during ischemia and was significantly increased at 12 h after reperfusion. Few TNFR1-like imuunoreactivity (TNFR1-LI) was observed in the cortex of normal animals. However, TNFR1-LI was increased at 1 h during ischemia, then it was decreased at 3-6 h, and was increased again at 12-24 h after reperfusion in the core of ischemic area. TNFR1-LI was demonstrated in both neurons and astrocytes but not in oligodendrocytes and microglia/macrophages at 24 h after reperfusion. At 96 h after tMCAO, TNFR1-LI was increased in the perifocal region and it appeared to be displayed the astrocyte-like cells. By use of double immunostaining method, we found that the ICAM-1-LI was overlapped with GFAP-LI. Our data indicates that the expression of TNFR1 is up-regulated in accordance with ischemic insult and delayed expressed TNFR1-LI co-localized with ICAM-1-LI in astrocytes after tMCAO. These results suggest that astroglial ICAM-1 is regulated by TNF-alpha dependent pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2',3'-Cyclic-Nucleotide...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Glial Fibrillary Acidic Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mtap2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Neurofilament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor...,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor Decoy...,
http://linkedlifedata.com/resource/pubmed/chemical/monocyte-macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/neurofilament protein H,
http://linkedlifedata.com/resource/pubmed/chemical/recombinant human tumor necrosis...
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0304-3940
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
370
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
30-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15489012-2',3'-Cyclic-Nucleotide Phosphodiesterases,
pubmed-meshheading:15489012-Animals,
pubmed-meshheading:15489012-Antigens, Differentiation,
pubmed-meshheading:15489012-Astrocytes,
pubmed-meshheading:15489012-Fluorescent Antibody Technique,
pubmed-meshheading:15489012-Functional Laterality,
pubmed-meshheading:15489012-Gene Expression Regulation,
pubmed-meshheading:15489012-Glial Fibrillary Acidic Protein,
pubmed-meshheading:15489012-Intercellular Adhesion Molecule-1,
pubmed-meshheading:15489012-Ischemic Attack, Transient,
pubmed-meshheading:15489012-Male,
pubmed-meshheading:15489012-Mice,
pubmed-meshheading:15489012-Microtubule-Associated Proteins,
pubmed-meshheading:15489012-Neurofilament Proteins,
pubmed-meshheading:15489012-RNA, Messenger,
pubmed-meshheading:15489012-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:15489012-Receptors, Tumor Necrosis Factor, Type I,
pubmed-meshheading:15489012-Reperfusion Injury,
pubmed-meshheading:15489012-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15489012-Time Factors,
pubmed-meshheading:15489012-Tumor Necrosis Factor Decoy Receptors
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pubmed:year |
2004
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pubmed:articleTitle |
Delayed expressed TNFR1 co-localize with ICAM-1 in astrocyte in mice brain after transient focal ischemia.
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pubmed:affiliation |
Department of Anatomy, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Japan Science and Technology, Tokyo 142-8555, Japan. yin@med.showa-u.ac.jp
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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