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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1992-4-17
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pubmed:abstractText |
The Drosophila melanogaster alcohol dehydrogenase (Adh) gene is expressed in a specific set of tissues during larval development and in adults. Expression in the adult fat body is controlled by the Adh adult enhancer (AAE). Previous studies identified a negative regulatory element in the AAE and a protein that binds specifically to this sequence [adult enhancer factor-1 (AEF-1)]. Here, we show that the AEF-1-binding site in the AAE and in two other Drosophila fat body enhancers overlaps a sequence recognized by the mammalian transcription factor CCAAT/enhancer-binding protein (C/EBP). Remarkably, these two proteins also bind specifically to overlapping sites in a liver-specific regulatory element of the human Adh gene. Cotransfection experiments in mammalian cells reveal that C/EBP stimulates the activity of the AAE by 50-fold, and this activity can be suppressed by AEF-1. In addition, AEF-1 prevents C/EBP binding in vitro, and displaces prebound C/EBP. Thus, a tissue-specific regulatory unit consisting of one positive and one negative regulatory element has been conserved between Drosophila and man.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0890-9369
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:geneSymbol |
Adh
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
454-65
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:1547943-Alcohol Dehydrogenase,
pubmed-meshheading:1547943-Animals,
pubmed-meshheading:1547943-Base Sequence,
pubmed-meshheading:1547943-Binding Sites,
pubmed-meshheading:1547943-Biological Evolution,
pubmed-meshheading:1547943-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:1547943-DNA,
pubmed-meshheading:1547943-DNA Fingerprinting,
pubmed-meshheading:1547943-DNA-Binding Proteins,
pubmed-meshheading:1547943-Drosophila Proteins,
pubmed-meshheading:1547943-Drosophila melanogaster,
pubmed-meshheading:1547943-Enhancer Elements, Genetic,
pubmed-meshheading:1547943-Fat Body,
pubmed-meshheading:1547943-Female,
pubmed-meshheading:1547943-Gene Expression,
pubmed-meshheading:1547943-Molecular Sequence Data,
pubmed-meshheading:1547943-Mutation,
pubmed-meshheading:1547943-Nuclear Proteins,
pubmed-meshheading:1547943-Ovary,
pubmed-meshheading:1547943-Promoter Regions, Genetic,
pubmed-meshheading:1547943-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1547943-Transcription, Genetic,
pubmed-meshheading:1547943-Transcription Factors
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pubmed:year |
1992
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pubmed:articleTitle |
A conserved regulatory unit implicated in tissue-specific gene expression in Drosophila and man.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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