Source:http://linkedlifedata.com/resource/pubmed/id/15475485
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2004-10-11
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pubmed:abstractText |
Oxidative stress is stated to be a central mechanism of hepatocellular injury in alcohol-induced liver injury. Recent reports have shown that Kupffer cell dysfunction in the leptin-deficient state contributes partly to the increased sensitivity to endotoxin liver injury. Here we report that leptin also plays a key role in the development of alcoholic liver injury and that leptin signaling in hepatocytes is involved in cellular mechanisms that mediate ethanol-induced oxidative stress. We found that chronic ethanol feeding in leptin receptor-deficient Zucker (fa/fa) rats for 6 wk resulted in a much more severe liver injury and augmented accumulation of hepatic lipid peroxidation compared with control littermates. The hepatic induction of stress-response and antioxidant proteins, such as metallothionein (MT)-1 and -2, was significantly suppressed in fa/fa rats after chronic ethanol feeding. Zinc concentration in liver was also decreased in fa/fa rats, compared with control littermates. In primary cultured hepatocytes from fa/fa rats, incubation with ethanol significantly suppressed MT-1 and -2 expressions. Addition of leptin to leptin-deficient ob/ob mouse primary hepatocytes led to an increase in MT-1 and -2 mRNA levels and a decrease in oxidative stress after incubation with ethanol. In conclusion, leptin deficiency enhances sensitivity of rats to alcohol-induced steatohepatitis through hepatocyte-specific interaction of MT-1 and -2 and resultant exaggeration of oxidative stress in hepatocytes. These findings suggest that leptin resistance in hepatocytes is an important mechanism of alcohol-induced liver injury.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ethanol,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Leptin,
http://linkedlifedata.com/resource/pubmed/chemical/Metallothionein,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Zinc,
http://linkedlifedata.com/resource/pubmed/chemical/metallothionein 2 protein, rat
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0193-1857
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pubmed:author |
pubmed-author:AndoSatoshiS,
pubmed-author:AzumaToshifumiT,
pubmed-author:HibiToshifumiT,
pubmed-author:InokuchiSayakaS,
pubmed-author:IshiiHiromasaH,
pubmed-author:KatoShinzoS,
pubmed-author:KitamuraNaotoN,
pubmed-author:NagataHiroshiH,
pubmed-author:NishimuraTakeshiT,
pubmed-author:TamiyaGenG,
pubmed-author:TomitaKengoK
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pubmed:issnType |
Print
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pubmed:volume |
287
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
G1078-85
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15475485-Animals,
pubmed-meshheading:15475485-Drug Administration Schedule,
pubmed-meshheading:15475485-Ethanol,
pubmed-meshheading:15475485-Fatty Liver,
pubmed-meshheading:15475485-Fluorescent Dyes,
pubmed-meshheading:15475485-Immunohistochemistry,
pubmed-meshheading:15475485-Kupffer Cells,
pubmed-meshheading:15475485-Leptin,
pubmed-meshheading:15475485-Male,
pubmed-meshheading:15475485-Metallothionein,
pubmed-meshheading:15475485-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:15475485-RNA, Messenger,
pubmed-meshheading:15475485-Rats,
pubmed-meshheading:15475485-Rats, Zucker,
pubmed-meshheading:15475485-Tumor Necrosis Factor-alpha,
pubmed-meshheading:15475485-Zinc
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pubmed:year |
2004
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pubmed:articleTitle |
Leptin deficiency enhances sensitivity of rats to alcoholic steatohepatitis through suppression of metallothionein.
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pubmed:affiliation |
Department of Internal Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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