Linked Life Data

15473329

Source:http://linkedlifedata.com/resource/pubmed/id/15473329

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2004-10-11
pubmed:abstractText
The strategies of large-scale mutagenesis and gene screening include chemical mutagenesis, insertional mutagenesis and gene trap. Insertional mutagenesis is a method for identifying genes by using the integration of DNA as the mutagen, thereby facilitating the cloning of the mutated gene. The use of retrovirus-mediated insertional mutagenesis in zebrafish has led to the mutation and rapid identification of hundreds of genes required for embryonic development and cell growth. Gene trap elements are also used in the construction of retrovirus vectors. The whole system is likely to make zebrafish the first vertebrate to achieve saturation mutagenesis and gene screening.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0379-4172
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
750-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
[Development of retrovirus-mediated insertional mutagenesis in zebrafish and its application in saturation mutagenesis and gene screening].
pubmed:affiliation
College of Life Science, Peking University, Beijing 100871, China. qifei@pku.edu.cn
pubmed:publicationType
Journal Article, English Abstract, Review, Research Support, Non-U.S. Gov't