Linked Life Data

15459777

Source:http://linkedlifedata.com/resource/pubmed/id/15459777

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-1-12
pubmed:abstractText
Connective tissue growth factor (CTGF) is a newly described protein that stimulates transforming growth factor-beta1 (TGF-beta1). We evaluated the expression of CTGF mRNA in operative biopsy specimens from biliary atresia (BA) patients and normal controls to assess the role of CTGF in BA. Liver biopsy specimens were taken from BA patients at the time of portoenterostomy (n=22) and compared with specimens taken from normal controls (n=6). In situ hybridization was used to stain CTGF mRNA in all specimens. The distribution of collagen type IV (C-IV) was also assessed in the same specimens as an indicator of the severity of fibrosis present at the time of biopsy. Results were analyzed to determine whether there was any correlation between CTGF and C-IV and outcome. Of the 22 postoperative BA patients, eventual outcome was good in 17 (group I), and five (group II) subsequently required liver transplantation. Control specimens (n=6) had no apparent CTGF mRNA expression, and median C-IV positive immunoreactivity was 1.23+/-0.25%. CTGF mRNA was weakly expressed in hepatic stellate cells (HSC) and hepatocytes in specimens from group I, and median C-IV-positive immunoreactivity was 3.18+/-0.86%. However, in specimens from group II, there was increased CTGF mRNA in HSC and hepatocytes. Median C-IV-positive immunoreactivity was 6.31+/-0.96%. There was a significant correlation between CTGF mRNA intensity and the amount of C-IV, which implies that CTGF expression reflects prognosis. This study provides the first evidence that CTGF is strongly expressed in BA, in particular in HSC and hepatocytes, suggesting that they may be a source of CTGF. The strong correlation with C-IV indicates that CTGF plays a major role in the pathogenesis of progressive fibrosis in BA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0179-0358
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12-6
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15459777-Bile Ducts, Intrahepatic, pubmed-meshheading:15459777-Biliary Atresia, pubmed-meshheading:15459777-Biological Markers, pubmed-meshheading:15459777-Biopsy, pubmed-meshheading:15459777-Child, pubmed-meshheading:15459777-Collagen Type IV, pubmed-meshheading:15459777-Connective Tissue Growth Factor, pubmed-meshheading:15459777-Disease Progression, pubmed-meshheading:15459777-Female, pubmed-meshheading:15459777-Follow-Up Studies, pubmed-meshheading:15459777-Gene Expression, pubmed-meshheading:15459777-Humans, pubmed-meshheading:15459777-Immediate-Early Proteins, pubmed-meshheading:15459777-Immunohistochemistry, pubmed-meshheading:15459777-In Situ Hybridization, pubmed-meshheading:15459777-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:15459777-Liver Cirrhosis, pubmed-meshheading:15459777-Male, pubmed-meshheading:15459777-Prognosis, pubmed-meshheading:15459777-RNA, Messenger, pubmed-meshheading:15459777-Transforming Growth Factor beta, pubmed-meshheading:15459777-Transforming Growth Factor beta1
pubmed:year
2005
pubmed:articleTitle
Connective tissue growth factor and progressive fibrosis in biliary atresia.
pubmed:affiliation
Department of Pediatric Surgery, Juntendo University School of Medicine, 2-1-1 Hongo, 113-8421 Bunkyo-ku, Tokyo, Japan. koba@med.juntendo.ac.jp
pubmed:publicationType
Journal Article, Comparative Study