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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5693
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pubmed:dateCreated |
2004-10-1
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pubmed:abstractText |
We show that activated collagenase (MMP-1) moves processively on the collagen fibril. The mechanism of movement is a biased diffusion with the bias component dependent on the proteolysis of its substrate, not adenosine triphosphate (ATP) hydrolysis. Inactivation of the enzyme by a single amino acid residue substitution in the active center eliminates the bias without noticeable effect on rate of diffusion. Monte Carlo simulations using a model similar to a "burnt bridge" Brownian ratchet accurately describe our experimental results and previous observations on kinetics of collagen digestion. The biological implications of MMP-1 acting as a molecular ratchet tethered to the cell surface suggest new mechanisms for its role in tissue remodeling and cell-matrix interaction.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1095-9203
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
306
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
108-11
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15459390-Adenosine Triphosphate,
pubmed-meshheading:15459390-Amino Acid Substitution,
pubmed-meshheading:15459390-Animals,
pubmed-meshheading:15459390-Collagen,
pubmed-meshheading:15459390-Computer Simulation,
pubmed-meshheading:15459390-Diffusion,
pubmed-meshheading:15459390-Fluorescence,
pubmed-meshheading:15459390-Humans,
pubmed-meshheading:15459390-Hydrolysis,
pubmed-meshheading:15459390-Mathematics,
pubmed-meshheading:15459390-Matrix Metalloproteinase 1,
pubmed-meshheading:15459390-Microscopy, Fluorescence,
pubmed-meshheading:15459390-Models, Chemical,
pubmed-meshheading:15459390-Molecular Motor Proteins,
pubmed-meshheading:15459390-Monte Carlo Method,
pubmed-meshheading:15459390-Point Mutation,
pubmed-meshheading:15459390-Protein Transport,
pubmed-meshheading:15459390-Rats,
pubmed-meshheading:15459390-Recombinant Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
Interstitial collagenase is a Brownian ratchet driven by proteolysis of collagen.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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