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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 21
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pubmed:dateCreated |
2004-9-30
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pubmed:abstractText |
Cysteine proteases are involved in the degradation of intracellular and extracellular proteins, although their precise roles in vivo are not well understood. Here we characterise a genetic mutant of the Caenorhabditis elegans cathepsin L protease gene cpl-1. CPL-1 is provided maternally and is essential for C. elegans embryogenesis. Immunofluorescence and electron microscopy data show that yolk endocytosis and initial yolk platelet formation occur normally in cpl-1 mutant oocytes and embryos. However, at around the 8-12 cell stage of embryogenesis, yolk platelets begin to aggregate and these enlarged yolk platelets fill the cytoplasm of cpl-1 mutant embryos. Coincident with this aggregation is loss of fluorescence from a yolk green fluorescent protein (YP170::GFP). This suggests that loss of CPL-1 activity leads to aberrant processing and/or conformational changes in yolk proteins, resulting in abnormal platelet fusion. This study has relevance to the abnormal fusion and aggregation of lysosomes in cathepsin L-deficient mice and to other lysosomal disorders.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9533
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
117
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5133-43
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15456850-Animals,
pubmed-meshheading:15456850-Blood Platelets,
pubmed-meshheading:15456850-Blotting, Western,
pubmed-meshheading:15456850-Caenorhabditis elegans,
pubmed-meshheading:15456850-Caenorhabditis elegans Proteins,
pubmed-meshheading:15456850-Cathepsin L,
pubmed-meshheading:15456850-Cathepsins,
pubmed-meshheading:15456850-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:15456850-Endocytosis,
pubmed-meshheading:15456850-Gene Expression Regulation, Developmental,
pubmed-meshheading:15456850-Green Fluorescent Proteins,
pubmed-meshheading:15456850-Lysosomes,
pubmed-meshheading:15456850-Microscopy, Electron,
pubmed-meshheading:15456850-Microscopy, Fluorescence,
pubmed-meshheading:15456850-Microscopy, Immunoelectron,
pubmed-meshheading:15456850-Mutation,
pubmed-meshheading:15456850-Phenotype,
pubmed-meshheading:15456850-Protein Conformation,
pubmed-meshheading:15456850-RNA Interference,
pubmed-meshheading:15456850-Time Factors,
pubmed-meshheading:15456850-Xenopus,
pubmed-meshheading:15456850-Yolk Sac
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pubmed:year |
2004
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pubmed:articleTitle |
Cathepsin L protease (CPL-1) is essential for yolk processing during embryogenesis in Caenorhabditis elegans.
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pubmed:affiliation |
Department of Veterinary Parasitology, Institute of Comparative Medicine, University of Glasgow, Garscube Estate, Bearsden Road, G61 1QH, UK. c.britton@vet.gla.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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