Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1992-3-24
pubmed:abstractText
Poly(A)+ mRNA was isolated from rabbit kidney cortex and injected into Xenopus laevis oocytes. Injection of mRNA resulted in a time- and dose-dependent increase in Na(+)-independent uptake of L-[3H]alanine and L-[3H]arginine. L-Alanine uptake was stimulated about 3-fold and L-arginine uptake was stimulated about 8-fold after injection of mRNA (25-50 ng, after 3-6 days) as compared with water-injected oocytes. T.I.C. of oocyte extracts suggested that the increased uptake actually represented an increase in the oocyte content of labelled L-alanine and L-arginine. The expressed L-alanine uptake, obtained by subtracting the uptake in water-injected oocytes from that in mRNA-injected oocytes, showed saturability and was inhibited completely by 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid (BCH) and L-arginine. The expressed L-arginine uptake in mRNA-injected oocytes also showed saturability, being completely inhibited by L-dibasic amino acids) and partially inhibited by BCH. Expression of both L-alanine and L-arginine uptake showed clear cis-inhibition by cationic (e.g. L-arginine) and neutral (e.g. L-leucine) amino acids. In all, this points to the expression of a Na(+)-independent transport system with broad specificity (i.e. b degree, (+)-like). In addition, part of the expressed uptake of L-arginine could be due to a system y(+)-like transporter. After size fractionation through a sucrose density gradient, the mRNA species encoding these increased transport activities (Na(+)-independent transport of L-alanine and of L-arginine) were found in fractions of an average mRNA chain-length of 1.8-2.4 kb. On the basis of these results, we conclude that Na(+)-independent transport system(s) for L-alanine and L-arginine from rabbit renal cortical tissues, most likely proximal tubules, are expressed in Xenopus laevis oocytes. These observations may represent the first steps towards expression and cloning of these transport pathways.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-1971509, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-2241165, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-2380194, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-2396979, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-2404290, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-2446136, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-2480748, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-2592432, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-3054116, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-3125176, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-3278739, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-3293095, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-3300747, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-3415246, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-5773053, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-6370115, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-6383473, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-6439715, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-6708092, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-6808139, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-6811749, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-7040384, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-7057450, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-7068644, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-7118928, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-7359578, http://linkedlifedata.com/resource/pubmed/commentcorrection/1536650-940260
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
281 ( Pt 3)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
717-23
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Expression of Na(+)-independent amino acid transport in Xenopus laevis oocytes by injection of rabbit kidney cortex mRNA.
pubmed:affiliation
Institute of Physiology, University of Zürich, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't