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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2004-9-14
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pubmed:abstractText |
Osteopetrotic mice lacking functional M-CSF recover with ageing, suggesting alternate osteoclastogenesis pathways exist. One alternative is GM-CSF, treatment with which improves the osteopetrosis. Our objective was to determine whether GM-CSF could replace M-CSF in human osteoclastogenesis in vitro. Human CFU-GM precursors cultured with RANKL differentiate into osteoclasts without added M-CSF, indicating constitutive production of M-CSF. Addition of M-CSF antibody completely inhibited differentiation, demonstrating M-CSF-dependence in vitro. Co-treatment with low concentrations (0.01 ng/mL) of GM-CSF for 14 days or higher concentrations (10 ng/mL) for the first 1-2 days enhanced osteoclastogenesis but this effect was blocked with M-CSF antibody. Treatment with GM-CSF transiently increased M-CSF mRNA expression at 3 h but suppressed expression at 7-14 days. Neither FLT3-ligand nor VEGF supported osteoclastogenesis in the absence of M-CSF. Thus, in vitro human osteoclastogenesis is dependent on M-CSF and the stimulatory effects of GM-CSF are mediated by M-CSF. Rescue by GM-CSF in M-CSF-deficiency is unlikely to be directly mediated by FLT3-ligand or VEGF.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Colony-Stimulating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RANK Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Activator of Nuclear...,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TNFRSF11A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TNFSF11 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfrsf11a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfsf11 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
321
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7-12
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15358207-Animals,
pubmed-meshheading:15358207-Base Sequence,
pubmed-meshheading:15358207-Carrier Proteins,
pubmed-meshheading:15358207-Colony-Forming Units Assay,
pubmed-meshheading:15358207-DNA Primers,
pubmed-meshheading:15358207-Fetal Blood,
pubmed-meshheading:15358207-Gene Expression Regulation,
pubmed-meshheading:15358207-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:15358207-Humans,
pubmed-meshheading:15358207-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:15358207-Membrane Glycoproteins,
pubmed-meshheading:15358207-Mice,
pubmed-meshheading:15358207-Osteoclasts,
pubmed-meshheading:15358207-Polymerase Chain Reaction,
pubmed-meshheading:15358207-RANK Ligand,
pubmed-meshheading:15358207-RNA, Messenger,
pubmed-meshheading:15358207-Receptor Activator of Nuclear Factor-kappa B,
pubmed-meshheading:15358207-Recombinant Fusion Proteins,
pubmed-meshheading:15358207-Recombinant Proteins,
pubmed-meshheading:15358207-Transcription, Genetic,
pubmed-meshheading:15358207-Vascular Endothelial Growth Factor A
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pubmed:year |
2004
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pubmed:articleTitle |
GM-CSF cannot substitute for M-CSF in human osteoclastogenesis.
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pubmed:affiliation |
Department of Clinical and Biomedical Sciences, Barwon Health, University of Melbourne, Geelong, Victoria 3220, Australia. j.hodgel@pgrad.unimelb.edu.au
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pubmed:publicationType |
Journal Article
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