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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2004-9-7
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182918,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182919,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182920,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182921,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182922,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182923,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182924,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182925,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182926,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182927,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182928,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182929,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182930,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182931,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182932,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182933,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182934,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182935,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182936,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182937,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB182938
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pubmed:abstractText |
Wild watermelon (Citrullus lanatus sp.) has the ability to tolerate severe drought/high light stress conditions despite carrying out normal C3-type photosynthesis. Here, mRNA differential display was employed to isolate drought-responsive genes in the leaves of wild watermelon. One of the isolated genes, CLMT2, shared significant homology with type-2 metallothionein (MT) sequences from other plants. The second-order rate constant for the reaction between a recombinant CLMT2 protein and hydroxyl radicals was estimated to be 1.2 x 10(11) M(-1) s(-1), demonstrating that CLMT2 had an extraordinary high activity for detoxifying hydroxyl radicals. Moreover, hydroxyl radical-catalyzed degradation of watermelon genomic DNA was effectively suppressed by CLMT2 in vitro. This is the first demonstration of a plant MT with antioxidant properties. The results suggest that CLMT2 induction contributes to the survival of wild watermelon under severe drought/high light stress conditions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arabidopsis Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxyl Radical,
http://linkedlifedata.com/resource/pubmed/chemical/MT2A protein, Arabidopsis,
http://linkedlifedata.com/resource/pubmed/chemical/Metallothionein,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2004 Elsevier Inc.
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
323
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
72-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15351703-Amino Acid Sequence,
pubmed-meshheading:15351703-Arabidopsis Proteins,
pubmed-meshheading:15351703-Catalysis,
pubmed-meshheading:15351703-Citrullus,
pubmed-meshheading:15351703-Cloning, Molecular,
pubmed-meshheading:15351703-DNA,
pubmed-meshheading:15351703-DNA, Complementary,
pubmed-meshheading:15351703-Disasters,
pubmed-meshheading:15351703-Dose-Response Relationship, Drug,
pubmed-meshheading:15351703-Gene Expression Profiling,
pubmed-meshheading:15351703-Hydroxyl Radical,
pubmed-meshheading:15351703-Metallothionein,
pubmed-meshheading:15351703-Molecular Sequence Data,
pubmed-meshheading:15351703-Oxidative Stress,
pubmed-meshheading:15351703-Phylogeny,
pubmed-meshheading:15351703-Plant Proteins,
pubmed-meshheading:15351703-RNA,
pubmed-meshheading:15351703-RNA, Messenger,
pubmed-meshheading:15351703-Recombinant Proteins,
pubmed-meshheading:15351703-Sequence Homology, Amino Acid,
pubmed-meshheading:15351703-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:15351703-Time Factors
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pubmed:year |
2004
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pubmed:articleTitle |
Potent hydroxyl radical-scavenging activity of drought-induced type-2 metallothionein in wild watermelon.
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pubmed:affiliation |
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0101, Japan. akashi@bs.naist.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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