|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017337,
umls-concept:C0019691,
umls-concept:C0023440,
umls-concept:C0025252,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0074479,
umls-concept:C0079382,
umls-concept:C0205250,
umls-concept:C0679058,
umls-concept:C1420364,
umls-concept:C1511695,
umls-concept:C1521871,
umls-concept:C1547699,
umls-concept:C2700640
|
|
pubmed:issue |
5
|
|
pubmed:dateCreated |
1992-5-28
|
|
pubmed:abstractText |
Using a differential hybridization method, we isolated a cDNA (melF) overexpressed in a murine Friend erythroleukemia cell line, F5-5. Southern blotting and sequence analysis revealed that the melF cDNA encodes a membrane protein of 395 amino acids that is the couterpart to human CD43 (leukosialin) surface antigen of lymphocytes in mice. The melF/mouse CD43 (mCD43) gene was found to be amplified eight- to 10-fold not only in F5-5 cells, but also in their sib cell lines, including the earliest clone established. This indicates that the amplification of melF/mCD43 gene occurred at an early stage of malignant transformation of the F5-5 family. Furthermore, the same gene was rearranged in another Friend erythroleukemia cell line TSA8. In both cell lines, F5-5 and TSA8, the levels of melF/mCD43 mRNA were 30- to 50-fold higher than that in uninfected spleen cells. Among normal tissues examined, the expression of melF/mCD43 gene was restricted to hematopoietic tissues. These results suggest that melF/mCD43 gene has an important role in progression of leukemic cells in the erythroid lineage and also has a physiological function in normal hematopoiesis.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD43,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Retroviridae Proteins, Oncogenic,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Spn protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/v-Spi-1 protein, Friend spleen...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0950-9232
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
7
|
|
pubmed:geneSymbol |
melF
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
919-26
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:1533277-Amino Acid Sequence,
pubmed-meshheading:1533277-Animals,
pubmed-meshheading:1533277-Antigens, CD,
pubmed-meshheading:1533277-Antigens, CD43,
pubmed-meshheading:1533277-Blotting, Northern,
pubmed-meshheading:1533277-Blotting, Southern,
pubmed-meshheading:1533277-Blotting, Western,
pubmed-meshheading:1533277-Cell Line,
pubmed-meshheading:1533277-Cell Transformation, Neoplastic,
pubmed-meshheading:1533277-Chromosome Mapping,
pubmed-meshheading:1533277-Cloning, Molecular,
pubmed-meshheading:1533277-DNA-Binding Proteins,
pubmed-meshheading:1533277-Gene Rearrangement,
pubmed-meshheading:1533277-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:1533277-Liver,
pubmed-meshheading:1533277-Mice,
pubmed-meshheading:1533277-Molecular Sequence Data,
pubmed-meshheading:1533277-Multigene Family,
pubmed-meshheading:1533277-Retroviridae Proteins, Oncogenic,
pubmed-meshheading:1533277-Sialoglycoproteins,
pubmed-meshheading:1533277-Spleen
|
|
pubmed:year |
1992
|
|
pubmed:articleTitle |
Amplification and rearrangement of melF/mouse CD43 (leukosialin) gene encoding a highly glycosylated membrane protein gp120 in Friend erythroleukemia cells.
|
|
pubmed:affiliation |
Department of Genetics, University of Tokyo, Japan.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
