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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1992-10-22
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pubmed:databankReference |
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pubmed:abstractText |
We report here the cDNA cloning of a putative gastrin receptor from enterochromaffin-like (ECL) carcinoid tumor of Mastomys natalensis. For this study, we used the polymerase chain reaction technique to amplify transmembrane domain sequences related to rat pancreatic cholecystokinin (CCK)-A receptor from the ECL tumor cDNA library. The amino acid sequence deduced from the cloned cDNA showed 85.7% and 49.0% identity to canine parietal cell gastrin receptor and rat pancreatic CCK-A receptor, respectively. Ligand binding studies using COS-7 cells transfected with the cDNA showed the same binding specificity for gastrin and CCK-8 as the gastrin receptor on the Mastomys carcinoid tumor membrane. Both gastrin and CCK-8 elevated free cytosolic calcium concentration in COS-7 cells expressing the cloned receptor. RNA blot analysis revealed the expression of the gastrin receptor in both Mastomys stomach and brain.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
187
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
1151-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1530611-Amino Acid Sequence,
pubmed-meshheading:1530611-Animals,
pubmed-meshheading:1530611-Base Sequence,
pubmed-meshheading:1530611-Calcium,
pubmed-meshheading:1530611-Carcinoid Tumor,
pubmed-meshheading:1530611-Cloning, Molecular,
pubmed-meshheading:1530611-DNA,
pubmed-meshheading:1530611-Dogs,
pubmed-meshheading:1530611-Female,
pubmed-meshheading:1530611-Gastrins,
pubmed-meshheading:1530611-Molecular Sequence Data,
pubmed-meshheading:1530611-Muridae,
pubmed-meshheading:1530611-Pancreas,
pubmed-meshheading:1530611-Parietal Cells, Gastric,
pubmed-meshheading:1530611-Rats,
pubmed-meshheading:1530611-Receptors, Cholecystokinin,
pubmed-meshheading:1530611-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1530611-Sincalide,
pubmed-meshheading:1530611-Stomach Neoplasms,
pubmed-meshheading:1530611-Transfection
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pubmed:year |
1992
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pubmed:articleTitle |
Cloning and characterization of gastrin receptor from ECL carcinoid tumor of Mastomys natalensis.
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pubmed:affiliation |
Department of Medicine, Kobe University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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