Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 17
pubmed:dateCreated
2004-8-2
pubmed:abstractText
Bulk DNA within the eukaryotic genome is torsionarily relaxed. However, unconstrained negative supercoils of DNA have been detected in few local domains of the genome through preferential binding of psoralen. To make a genome-wide survey for such domains, we introduced biotinylated psoralen into Drosophila salivary glands and visualized it on polytene chromosomes with fluorescent streptavidin. We observed bright psoralen signals on many transcriptionally active interbands and puffs. Upon heat shock, the signals appeared on heat-shock puffs. The signals were resistant to RNase treatment but disappeared or became faint by previous nicking of DNA or inhibition of transcription with alpha-amanitin. These data show that transcription-coupled, unconstrained negative supercoils of DNA exist in approximately 150 loci within the interphase genome.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
117
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3797-805
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15252118-Amanitins, pubmed-meshheading:15252118-Animals, pubmed-meshheading:15252118-Biotinylation, pubmed-meshheading:15252118-Blotting, Southern, pubmed-meshheading:15252118-Chromosomes, pubmed-meshheading:15252118-DNA, pubmed-meshheading:15252118-DNA, Superhelical, pubmed-meshheading:15252118-Dose-Response Relationship, Drug, pubmed-meshheading:15252118-Drosophila melanogaster, pubmed-meshheading:15252118-Ficusin, pubmed-meshheading:15252118-Genome, pubmed-meshheading:15252118-Hot Temperature, pubmed-meshheading:15252118-Microscopy, Fluorescence, pubmed-meshheading:15252118-Models, Genetic, pubmed-meshheading:15252118-Protein Binding, pubmed-meshheading:15252118-Protein Structure, Tertiary, pubmed-meshheading:15252118-RNA, pubmed-meshheading:15252118-Ribonucleases, pubmed-meshheading:15252118-Transcription, Genetic, pubmed-meshheading:15252118-X-Rays
pubmed:year
2004
pubmed:articleTitle
Visualization of unconstrained negative supercoils of DNA on polytene chromosomes of Drosophila.
pubmed:affiliation
Department of Developmental Genetics, National Institute of Genetics, SOKENDAI, 1111 Yata, Mishima, Shizuokaken 411-8540, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't