Source:http://linkedlifedata.com/resource/pubmed/id/15242735
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2004-7-9
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| pubmed:abstractText |
The role of tumor necrosis factor (TNF)-alpha in myocardial ischemia/reperfusion injury remains controversial. We used homozygous TNF-alpha null mice (TNF-alpha(-/-)) to determine whether TNF-alpha modulates myocardial ischemia/reperfusion injury. Mice were subjected to a 30-min coronary occlusion followed by 24 h of reperfusion. When wild-type mice were preconditioned with six cycles of 4-min coronary occlusion/4-min reperfusion 24 h before the 30-min occlusion, infarct size was reduced from 58.6 +/- 1.9% of the risk region to 19.3 +/- 3.6%, indicating a late preconditioning (PC) effect. In non-preconditioned TNF-alpha(-/-) mice, infarct size was similar to that observed in wild-type mice (55.5 +/- 3.7%). However, in TNF-alpha(-/-) mice preconditioned with six occlusion/reperfusion cycles 24 h earlier, infarct size was not reduced (55.2 +/- 5.7%), indicating that the late PC protection against infarction was completely abolished. While minimal TNF-alpha immunoreactivity was detected in sham-operated hearts, extensive TNF-alpha expression was noted in the cytoplasm of cardiomyocytes in the ischemic/reperfused region 30 min after the PC ischemia. At 30 min after PC, wild-type mice exhibited increased DNA-binding activity of nuclear factor-kappa B (NF-kappa B) and activator protein-1 (AP-1) and nuclear translocation of p65, c-Jun and c-Fos; all of these changes were absent in TNF-alpha(-/-) mice. These data demonstrate that TNF-alpha does not modulate infarct size in the naïve (non-preconditioned) state but is essential for the development of the late phase of ischemic PC, possibly via the activation of NF-kappa B and AP-1 transcription factors.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
0022-2828
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| pubmed:author |
pubmed-author:BolliRobertoR,
pubmed-author:DawnBuddhadebB,
pubmed-author:GuoYiruY,
pubmed-author:HuntGregG,
pubmed-author:RezazadehArashA,
pubmed-author:SteinAdam BAB,
pubmed-author:TanWeiW,
pubmed-author:VarmaJaiJ,
pubmed-author:WangOu-LiOL,
pubmed-author:WuWen-JianWJ,
pubmed-author:XuanYu-TingYT,
pubmed-author:ZhuXiaopingX
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| pubmed:issnType |
Print
|
| pubmed:volume |
37
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
51-61
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15242735-Active Transport, Cell Nucleus,
pubmed-meshheading:15242735-Animals,
pubmed-meshheading:15242735-Blotting, Western,
pubmed-meshheading:15242735-Cell Nucleus,
pubmed-meshheading:15242735-Cytoplasm,
pubmed-meshheading:15242735-Cytosol,
pubmed-meshheading:15242735-DNA,
pubmed-meshheading:15242735-Hemodynamics,
pubmed-meshheading:15242735-Homozygote,
pubmed-meshheading:15242735-Immunohistochemistry,
pubmed-meshheading:15242735-Ischemia,
pubmed-meshheading:15242735-Ischemic Preconditioning, Myocardial,
pubmed-meshheading:15242735-Mice,
pubmed-meshheading:15242735-Mice, Transgenic,
pubmed-meshheading:15242735-Myocardial Ischemia,
pubmed-meshheading:15242735-Myocardium,
pubmed-meshheading:15242735-Myocytes, Cardiac,
pubmed-meshheading:15242735-NF-kappa B,
pubmed-meshheading:15242735-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:15242735-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:15242735-Reperfusion Injury,
pubmed-meshheading:15242735-Risk,
pubmed-meshheading:15242735-Signal Transduction,
pubmed-meshheading:15242735-Temperature,
pubmed-meshheading:15242735-Time Factors,
pubmed-meshheading:15242735-Transcription Factor AP-1,
pubmed-meshheading:15242735-Tumor Necrosis Factor-alpha
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| pubmed:year |
2004
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| pubmed:articleTitle |
Tumor necrosis factor-alpha does not modulate ischemia/reperfusion injury in naïve myocardium but is essential for the development of late preconditioning.
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| pubmed:affiliation |
Experimental Research Laboratory, Division of Cardiology, University of Louisville, and the Jewish Hospital Heart and Lung Institute, Louisville, KY 40292, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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