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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2004-9-13
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pubmed:abstractText |
TNF-alpha has numerous biological activities, including the induction of chemokine expression, and is involved in many gastric injuries. C-C chemokines [monocyte chemotactic protein (MCP)-1 and macrophage inflammatory protein (MIP)-1alpha] and C-X-C chemokines [MIP-2 and cytokine-induced neutrophil chemoattractant (CINC)-2alpha] mediate chemotaxis of monocytes and neutrophils, respectively. We examined the roles of TNF-alpha and dynamics of chemokine expression in gastric ulceration including ulcer recurrence and indomethacin-induced injury. Rats with healed chronic gastric ulcers received intraperitoneal TNF-alpha to induce ulcer recurrence. Some rats were given neutralizing antibodies against neutrophils or MCP-1 together with TNF-alpha. In a separate experiment, rats were orally administered 20 mg/kg indomethacin with or without pretreatment with pentoxifylline (an inhibitor of TNF-alpha synthesis) or anti-MCP-1 antibody. TNF-alpha (1 microg/kg) induced gastric ulcer recurrence after 48 h, which was completely prevented by anti-neutrophil antibody. TNF-alpha increased the number of macrophages and MCP-1 mRNA expression in scarred mucosa from 4 h, whereas it increased MPO activities (marker of neutrophil infiltration) and mRNA expression of MIP-2 and CINC-2alpha from 24 h. Anti-MCP-1 antibody inhibited leukocyte infiltration with reduction of the levels of C-X-C chemokines and prevented ulcer recurrence. Indomethacin treatment increased TNF-alpha/chemokine mRNA expression from 30 min and induced macroscopic erosions after 4 h. Pentoxifylline inhibited the indomethacin-induced gastric injury with reduction of neutrophil infiltration and expression of chemokine (MCP-1, MIP-2, and CINC-2alpha). Anti-MCP-1 antibody also inhibited the injury and these inflammatory responses but did not affect TNF-alpha mRNA expression. In conclusion, increased MCP-1 triggered by TNF-alpha may play a key role in gastric ulceration by regulating leukocyte recruitment and chemokine expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-Pyridinylmethylsulfinylbenzimidazo...,
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents...,
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Ulcer Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Benzimidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL2,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL3,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL4,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL2,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcl2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcl2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Indomethacin,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Inflammatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Omeprazole,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/rabeprazole
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0193-1857
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
287
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
G919-28
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:15205118-2-Pyridinylmethylsulfinylbenzimidazoles,
pubmed-meshheading:15205118-Animals,
pubmed-meshheading:15205118-Anti-Inflammatory Agents, Non-Steroidal,
pubmed-meshheading:15205118-Anti-Ulcer Agents,
pubmed-meshheading:15205118-Antibodies,
pubmed-meshheading:15205118-Antineoplastic Agents,
pubmed-meshheading:15205118-Benzimidazoles,
pubmed-meshheading:15205118-Cell Movement,
pubmed-meshheading:15205118-Chemokine CCL2,
pubmed-meshheading:15205118-Chemokine CCL3,
pubmed-meshheading:15205118-Chemokine CCL4,
pubmed-meshheading:15205118-Chemokine CXCL2,
pubmed-meshheading:15205118-Chemokines,
pubmed-meshheading:15205118-Chemokines, CXC,
pubmed-meshheading:15205118-Gastric Acid,
pubmed-meshheading:15205118-Gastric Mucosa,
pubmed-meshheading:15205118-Indomethacin,
pubmed-meshheading:15205118-Intercellular Adhesion Molecule-1,
pubmed-meshheading:15205118-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:15205118-Leukocytes,
pubmed-meshheading:15205118-Macrophage Inflammatory Proteins,
pubmed-meshheading:15205118-Male,
pubmed-meshheading:15205118-Omeprazole,
pubmed-meshheading:15205118-RNA, Messenger,
pubmed-meshheading:15205118-Rats,
pubmed-meshheading:15205118-Rats, Wistar,
pubmed-meshheading:15205118-Recurrence,
pubmed-meshheading:15205118-Specific Pathogen-Free Organisms,
pubmed-meshheading:15205118-Stomach Ulcer,
pubmed-meshheading:15205118-Tumor Necrosis Factor-alpha
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pubmed:year |
2004
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pubmed:articleTitle |
Monocyte chemotactic protein-1 regulates leukocyte recruitment during gastric ulcer recurrence induced by tumor necrosis factor-alpha.
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pubmed:affiliation |
Dept. of Gastroenterology, Osaka City Univ. Graduate School of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka 545-8585, Japan. watanabet@med.osaka-cu.ac.jp
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pubmed:publicationType |
Journal Article
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