Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1992-10-6
pubmed:abstractText
Multiple HIV-1 nef genes were cloned from lymphocyte DNA of asymptomatic seropositive individuals by polymerase chain reaction (PCR). Sequence analysis of these clones revealed a unique set of nef variants with premature terminations (PCRnef 1 and 6), mutations at sites of potential posttranslational modification (PCRnef 2 and 3) and deletions. In common with laboratory isolates of nef, strong sequence conservation was observed in the central domain of nef and in the myristylation target sequence, with variable domains toward the N- and C-termini of the molecule. The biochemical function of nef remains elusive however, as the products of these genes cloned into a bacterial expression system failed to reveal any nucleotide binding activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0889-2229
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:geneSymbol
nef
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
537-43
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:1515209-Amino Acid Sequence, pubmed-meshheading:1515209-Cloning, Molecular, pubmed-meshheading:1515209-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:1515209-Escherichia coli, pubmed-meshheading:1515209-Gene Products, nef, pubmed-meshheading:1515209-Genes, nef, pubmed-meshheading:1515209-Genetic Variation, pubmed-meshheading:1515209-Glutathione Transferase, pubmed-meshheading:1515209-HIV Seropositivity, pubmed-meshheading:1515209-HIV-1, pubmed-meshheading:1515209-Humans, pubmed-meshheading:1515209-Molecular Sequence Data, pubmed-meshheading:1515209-Nucleotides, pubmed-meshheading:1515209-Polymerase Chain Reaction, pubmed-meshheading:1515209-Protein Binding, pubmed-meshheading:1515209-Recombinant Fusion Proteins, pubmed-meshheading:1515209-Sequence Alignment, pubmed-meshheading:1515209-nef Gene Products, Human Immunodeficiency Virus
pubmed:year
1992
pubmed:articleTitle
In vivo derived HIV-1 nef gene products are heterogeneous and lack detectable nucleotide binding activity.
pubmed:affiliation
Department of Veterinary Pathology, University of Glasgow, Scotland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't