Linked Life Data

15148565

Source:http://linkedlifedata.com/resource/pubmed/id/15148565

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2004-11-1
pubmed:abstractText
Tendonitis and tendon rupture have been reported to occur during or following therapy with fluoroquinolone antibiotics. Though the pathogenesis is unknown, several studies suggest that fluoroquinolone antibiotics alter proteoglycan content in soft tissues, including tendons, and thereby alter collagen fibrillogenesis. To better understand the mechanism of action of fluoroquinolones, we studied the effects of enrofloxacin, a widely used fluoroquinolone in veterinary medicine, on avian tendon cell cultures established from gastrocnemius tendons from 18-day-old chicken embryos. We found that cell proliferation was progressively inhibited with increasing concentrations of enrofloxacin. This was accompanied by changes in morphology, extracellular matrix content and collagen fibril formation as detected by electron microscopy. We also observed a 35% decrease in the content of total monosaccharides in enrofloxacin-treated cells. The ratio of individual monosaccharides was also altered in enrofloxacin-treated cells. Enrofloxacin also induced the synthesis of small amounts of keratan sulfate in tendon cells. Moreover we observed enrofloxacin-induced changes in glycosylation of decorin, the most abundant tendon proteoglycan, resulting in the emergence of multiple lower molecular bands that were identifiable as decorin after chondroitinase ABC and N-glycanase treatment of extracts from enrofloxacin-treated cells. Medium conditioned by enrofloxacin-treated cells contained less decorin than did medium conditioned by control cells. We hypothesize that enrofloxacin induces either changes in the number of N-linked oligosaccharides attached to the core protein of decorin or changes in decorin degradation process. In conclusion, our data suggest that enrofloxacin affects cell proliferation and extracellular matrix through changes in glycosylation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0340-5761
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
599-608
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:15148565-Animals, pubmed-meshheading:15148565-Antineoplastic Agents, pubmed-meshheading:15148565-Cell Proliferation, pubmed-meshheading:15148565-Cells, Cultured, pubmed-meshheading:15148565-Chick Embryo, pubmed-meshheading:15148565-Culture Media, Conditioned, pubmed-meshheading:15148565-Decorin, pubmed-meshheading:15148565-Dose-Response Relationship, Drug, pubmed-meshheading:15148565-Extracellular Matrix, pubmed-meshheading:15148565-Extracellular Matrix Proteins, pubmed-meshheading:15148565-Fluoroquinolones, pubmed-meshheading:15148565-Gas Chromatography-Mass Spectrometry, pubmed-meshheading:15148565-Glycosaminoglycans, pubmed-meshheading:15148565-Keratan Sulfate, pubmed-meshheading:15148565-Muscle, Skeletal, pubmed-meshheading:15148565-Proteoglycans, pubmed-meshheading:15148565-Quinolones, pubmed-meshheading:15148565-Reticulin, pubmed-meshheading:15148565-Tendons
pubmed:year
2004
pubmed:articleTitle
The effects of enrofloxacin on decorin and glycosaminoglycans in avian tendon cell cultures.
pubmed:affiliation
Department of Pathology, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't