|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1-3
|
|
pubmed:dateCreated |
2004-5-18
|
|
pubmed:abstractText |
Neurosteroids are known as allosteric modulators of ionotropic gamma-aminobutyric acid (GABA) receptors. Here, we investigated sites of positive allosteric modulation by allotetrahydrodeoxycorticosterone (5alpha-THDOC) at GABA receptors using the technique of chimeragenesis and the Xenopus oocyte expression system. Our findings have demonstrated that the region from transmembrane segment (TM) 4 to the C-terminus of the GABA(A) receptor alpha1 subunit is crucial for the action of 5alpha-THDOC, but insufficient for the action of another neurosteroid allopregnanolone, suggesting that a specific region critical for neurosteroid action at GABA receptors exists in the domain between TM4 and the C-terminus of GABA receptor subunits.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
21
|
|
pubmed:volume |
566
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
213-7
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:15147897-Allosteric Regulation,
pubmed-meshheading:15147897-Allosteric Site,
pubmed-meshheading:15147897-Amino Acid Sequence,
pubmed-meshheading:15147897-Animals,
pubmed-meshheading:15147897-Conserved Sequence,
pubmed-meshheading:15147897-Desoxycorticosterone,
pubmed-meshheading:15147897-Dose-Response Relationship, Drug,
pubmed-meshheading:15147897-Electrophysiology,
pubmed-meshheading:15147897-Female,
pubmed-meshheading:15147897-GABA-A Receptor Agonists,
pubmed-meshheading:15147897-Humans,
pubmed-meshheading:15147897-Molecular Sequence Data,
pubmed-meshheading:15147897-Oocytes,
pubmed-meshheading:15147897-Pregnanolone,
pubmed-meshheading:15147897-Protein Subunits,
pubmed-meshheading:15147897-Receptors, GABA-A,
pubmed-meshheading:15147897-Recombinant Proteins,
pubmed-meshheading:15147897-Xenopus Proteins,
pubmed-meshheading:15147897-Xenopus laevis
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
Sites of positive allosteric modulation by neurosteroids on ionotropic gamma-aminobutyric acid receptor subunits.
|
|
pubmed:affiliation |
Department of Pharmacology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishiku, Kitakyushu, Fukuoka 807-8555, Japan. susumu@med.uoeh-u.ac.jp
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
