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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2004-5-18
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pubmed:abstractText |
This study examined the effects of hypomorphic p75 neurotrophin receptor (p75NTR) expression and high levels of nerve growth factor (NGF) on trkA phosphorylation and downstream activation of p44/42 mitogen-activated protein kinase (MAPK). Post-ganglionic sympathetic neurons from postnatal day 1 p75NTR exon III null mutant (p75(-/-)) and 129/SvJ mice were cultured in the presence of 50 ng/mL NGF and analysed by Western blotting. Levels of phosphorylated trkA are increased in p75(-/-) neurons compared with 129/SvJ neurons, and these higher levels are maintained with continuous exposure to NGF. MAPK is also phosphorylated to a greater extent in p75(-/-) neurons than in 129/SvJ neurons, both within 10 min of exposure to NGF, and with continuous NGF treatment for 5 days. These data provide new insight into the mechanism underlying enhanced neurite outgrowth in p75(-/-) neurons, demonstrating that trkA and MAPK signalling in sympathetic neurons are increased when p75NTR function is disrupted.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0953-816X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2903-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15147324-Animals,
pubmed-meshheading:15147324-Animals, Newborn,
pubmed-meshheading:15147324-Blotting, Western,
pubmed-meshheading:15147324-Carrier Proteins,
pubmed-meshheading:15147324-Cell Count,
pubmed-meshheading:15147324-Cells, Cultured,
pubmed-meshheading:15147324-Membrane Proteins,
pubmed-meshheading:15147324-Mice,
pubmed-meshheading:15147324-Mice, Inbred Strains,
pubmed-meshheading:15147324-Mice, Knockout,
pubmed-meshheading:15147324-Mitogen-Activated Protein Kinases,
pubmed-meshheading:15147324-Nerve Growth Factor,
pubmed-meshheading:15147324-Neurons,
pubmed-meshheading:15147324-Phosphorylation,
pubmed-meshheading:15147324-Precipitin Tests,
pubmed-meshheading:15147324-Rats,
pubmed-meshheading:15147324-Receptor, Nerve Growth Factor,
pubmed-meshheading:15147324-Receptor, trkA,
pubmed-meshheading:15147324-Receptors, Nerve Growth Factor,
pubmed-meshheading:15147324-Superior Cervical Ganglion,
pubmed-meshheading:15147324-Time Factors
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pubmed:year |
2004
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pubmed:articleTitle |
TrkA and mitogen-activated protein kinase phosphorylation are enhanced in sympathetic neurons lacking functional p75 neurotrophin receptor expression.
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pubmed:affiliation |
Department of Anatomy and Cell Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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