Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
2004-5-14
pubmed:abstractText
Sub-micron-sized ultrahigh molecular-weight polyethylene (PE) debris is generated in the joint space as a result of articulation and cyclic loading of an orthopaedic implant. Its characterization requires isolation and subsequent analysis by ultra-structural methods. An innovative method based on the digestion of paraffin-embedded tissue samples was proposed. Tissue slices were digested with sodium hypochlorite directly on polycarbonate filter. The same procedure could be applied also to fresh synovial fluid. Plastic particles were not lost or damaged during treatment. Chemical identification of particles was done by micro-Raman spectroscopy that confirmed purity of retrieved PE particles. Size and shape of PE particles were characterised using scanning electron microscopy and were comparable in number and morphology to the retrieval by other authors. Equivalent diameter ranged from 0.48 to 0.95microm and particle number ranged from 9 to 23x10(9)/cm(3).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0142-9612
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5531-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
A new method for isolation of polyethylene wear debris from tissue and synovial fluid.
pubmed:affiliation
Laboratorio di Tecnologia Medica, Istituti Ortopedici Rizzoli, Via di Barbiano, 1/10, Bologna 40136, Italy. visentin@tecno.ior.it
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't