Linked Life Data

15121164

Source:http://linkedlifedata.com/resource/pubmed/id/15121164

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-5-3
pubmed:abstractText
FuGENE 6 Transfection Reagent has been commercially available since 1997. Since that time, its popularity has increased due to its ease of use, minimal to no cytotoxicity, and the high level of transfection in many different cell lines. FuGENE 6 Transfection Reagent is gentle on the cells. Adherent cells can be trypsinized and transfected by the DNA:FuGENE 6 reagent complex prior to plating, making it a strong candidate for high throughput applications. Additionally, low cell numbers can be transfected in 96-well plates. As with most reagents, the complex formation step is critical and special handling is required because the reagent is supplied in 80% ethanol. For example, contact with plastic must be avoided as inhibitors of transfection can leach from some plastics. We investigated parameters that have been reported to affect the transfection efficiency including the use of common antibiotics, passage level of the cells, and length of time for complex formation. These parameters are often cell line dependent and can be optimized to increase transfection efficiency for a specific cell line.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1046-2023
pubmed:author
pubmed:copyrightInfo
Copyright 2003 Elsevier Inc.
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
104-12
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
FuGENE 6 Transfection Reagent: the gentle power.
pubmed:affiliation
Roche Applied Science, Roche Diagnostics Corporation, 9115 Hague Road, Indianapolis, IN 46250-0414, USA. linda.jacobsen@roche.com
pubmed:publicationType
Journal Article