Linked Life Data

15115721

Source:http://linkedlifedata.com/resource/pubmed/id/15115721

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-8-23
pubmed:abstractText
In the present study, we cloned full-length cDNAs for epidermal growth factor (EGF), EGF receptor (EGFR), and three truncated forms of EGFR (EGFR15, 12, and 8) from the zebrafish ovary. Zebrafish EGF was predominantly expressed in the ovary and testis, while EGFR and its truncated forms were highly expressed in all tissues examined except the liver. In the ovary, the expression of EGF seemed to be more abundant in the follicles of early stages, while EGFR had much higher expression levels at later stages. Interestingly, although EGF was expressed in both the follicle cells and oocytes, its expression level was significantly higher in the oocytes. However, the expression of EGFR was mainly restricted to the follicle cells with little expression in the oocytes. The unique spatial patterns of EGF and EGFR expression within the follicle suggest that EGF may serve as a messenger from the oocyte to signal the follicle cells. EGF strongly stimulated the expression of both activin beta A and beta B, while it suppressed basal and hCG-induced follistatin expression in cultured follicle cells. These results, together with the evidence that EGF was predominantly expressed in the oocytes whereas EGFR was expressed in the follicle cells, strongly suggest that EGF is likely a potential paracrine/juxtacrine factor from the oocytes to regulate the function of the follicle cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
749-60
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15115721-Amino Acid Sequence, pubmed-meshheading:15115721-Animals, pubmed-meshheading:15115721-Cells, Cultured, pubmed-meshheading:15115721-Cloning, Molecular, pubmed-meshheading:15115721-Epidermal Growth Factor, pubmed-meshheading:15115721-Female, pubmed-meshheading:15115721-Follistatin, pubmed-meshheading:15115721-Gene Expression, pubmed-meshheading:15115721-Gonadotropins, pubmed-meshheading:15115721-Inhibin-beta Subunits, pubmed-meshheading:15115721-Male, pubmed-meshheading:15115721-Molecular Sequence Data, pubmed-meshheading:15115721-Oocytes, pubmed-meshheading:15115721-Ovarian Follicle, pubmed-meshheading:15115721-Paracrine Communication, pubmed-meshheading:15115721-Receptor, Epidermal Growth Factor, pubmed-meshheading:15115721-Sexual Maturation, pubmed-meshheading:15115721-Testis, pubmed-meshheading:15115721-Zebrafish
pubmed:year
2004
pubmed:articleTitle
Cloning of epidermal growth factor (EGF) and EGF receptor from the zebrafish ovary: evidence for EGF as a potential paracrine factor from the oocyte to regulate activin/follistatin system in the follicle cells.
pubmed:affiliation
Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't