Linked Life Data

15090600

Source:http://linkedlifedata.com/resource/pubmed/id/15090600

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 10
pubmed:dateCreated
2004-4-19
pubmed:abstractText
The identification and characterization of scaffold and targeting proteins of the ERK/MAP kinase pathway is important to understand the function and intracellular organization of this pathway. The F-actin binding protein leukocyte-specific protein 1 (LSP1) binds to PKCbetaI and expression of B-LSP1, an LSP1 truncate containing the PKCbetaI binding residues, inhibits anti-IgM-induced translocation of PKCbetaI to the plasma membrane and anti-IgM-induced activation of ERK2. To understand the role of LSP1 in the regulation of PKCbetaI-dependent ERK2 activation, we investigated whether LSP1 interacts with ERK/MAP kinase pathway components and targets these proteins to the actin cytoskeleton. We show that LSP1 associates with the ERK scaffold protein KSR and with MEK1 and ERK2. LSP1-associated MEK1 is activated by anti-IgM treatment and this activation is inhibited by expression of B-LSP1, suggesting that the activation of LSP1-associated MEK1 is PKCbetaI dependent. Confocal microscopy showed that LSP1 targets KSR, MEK1 and ERK2 to peripheral actin filaments. Thus our data show that LSP1 is a cytoskeletal targeting protein for the ERK/MAP kinase pathway and support a model in which MEK1 and ERK2 are organized in a cytoskeletal signaling complex together with KSR, PKCbetaI and LSP1 and are activated by anti-IgM in a PKCbetaI-dependent manner.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
117
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2151-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15090600-Actins, pubmed-meshheading:15090600-Blood Proteins, pubmed-meshheading:15090600-Carrier Proteins, pubmed-meshheading:15090600-Cell Line, pubmed-meshheading:15090600-Cell Line, Tumor, pubmed-meshheading:15090600-Cell Membrane, pubmed-meshheading:15090600-Cytoskeleton, pubmed-meshheading:15090600-Glutathione Transferase, pubmed-meshheading:15090600-Humans, pubmed-meshheading:15090600-Immunoglobulin M, pubmed-meshheading:15090600-Immunoprecipitation, pubmed-meshheading:15090600-Leukocytes, pubmed-meshheading:15090600-Lymphoma, B-Cell, pubmed-meshheading:15090600-MAP Kinase Kinase 1, pubmed-meshheading:15090600-MAP Kinase Signaling System, pubmed-meshheading:15090600-Membrane Proteins, pubmed-meshheading:15090600-Microfilament Proteins, pubmed-meshheading:15090600-Microscopy, Confocal, pubmed-meshheading:15090600-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:15090600-Protein Binding, pubmed-meshheading:15090600-Time Factors, pubmed-meshheading:15090600-Transfection
pubmed:year
2004
pubmed:articleTitle
Leukocyte-specific protein 1 targets the ERK/MAP kinase scaffold protein KSR and MEK1 and ERK2 to the actin cytoskeleton.
pubmed:affiliation
Cell Biology Programme, The Hospital for Sick Children Research Institute, Toronto, Ontario, M5G 1X8, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't