|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2004-4-6
|
|
pubmed:abstractText |
The RNase III enzyme Dicer processes RNA into siRNAs and miRNAs, which direct a RNA-induced silencing complex (RISC) to cleave mRNA or block its translation (RNAi). We have characterized mutations in the Drosophila dicer-1 and dicer-2 genes. Mutation in dicer-1 blocks processing of miRNA precursors, whereas dicer-2 mutants are defective for processing siRNA precursors. It has been recently found that Drosophila Dicer-1 and Dicer-2 are also components of siRNA-dependent RISC (siRISC). We find that Dicer-1 and Dicer-2 are required for siRNA-directed mRNA cleavage, though the RNase III activity of Dicer-2 is not required. Dicer-1 and Dicer-2 facilitate distinct steps in the assembly of siRISC. However, Dicer-1 but not Dicer-2 is essential for miRISC-directed translation repression. Thus, siRISCs and miRISCs are different with respect to Dicers in Drosophila.
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DCR-2 protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MicroRNAs,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Induced Silencing Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonuclease III
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0092-8674
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
2
|
|
pubmed:volume |
117
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
69-81
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:15066283-Animals,
pubmed-meshheading:15066283-Drosophila Proteins,
pubmed-meshheading:15066283-Drosophila melanogaster,
pubmed-meshheading:15066283-Eye Abnormalities,
pubmed-meshheading:15066283-Female,
pubmed-meshheading:15066283-Male,
pubmed-meshheading:15066283-MicroRNAs,
pubmed-meshheading:15066283-Molecular Sequence Data,
pubmed-meshheading:15066283-Mutation,
pubmed-meshheading:15066283-Protein Biosynthesis,
pubmed-meshheading:15066283-Protein Structure, Tertiary,
pubmed-meshheading:15066283-RNA, Messenger,
pubmed-meshheading:15066283-RNA, Small Interfering,
pubmed-meshheading:15066283-RNA Helicases,
pubmed-meshheading:15066283-RNA Interference,
pubmed-meshheading:15066283-RNA-Induced Silencing Complex,
pubmed-meshheading:15066283-Ribonuclease III,
pubmed-meshheading:15066283-Sequence Homology, Amino Acid
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways.
|
|
pubmed:affiliation |
Department of Biochemistry, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
