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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2004-4-5
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pubmed:abstractText |
We examined the effect of lidocaine on phosphorylation of the tyrosine kinase focal adhesion kinase (PP125FAK) in rat hippocampal slices by immunoblotting with both antiphosphotyrosine and specific anti-PP125FAK antibodies in the presence of tetrodotoxin (1 microM). Lidocaine induced a concentration-related increase in tyrosine phosphorylation of the 125-kDa band corresponding to PP125FAK phosphorylation (EC50 value=0.39+/-0.09 microM, maximal effect=169+/-28% of control, P<0.001). This effect was sensitive to neither the N-methyl-D-aspartate (NMDA) receptor antagonist dizocilpine (MK 801, 10 microM) nor the inhibitor of the ryanodine receptor dantrolene (30 microM). In contrast, it was completely blocked by the protein kinase C (PKC) inhibitors chelerythrin, bisindolylmaleimide I (GF 109203X) and bisindolylmaleimide IX (RO-318220, 10 microM). We conclude that lidocaine increases phosphorylation of the tyrosine kinase PP125FAK in the rat hippocampus by a tetrotoxin (TTX)-insensitive mechanism which involves activation of PKC.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anesthetics, Local,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Protein-Tyrosine...,
http://linkedlifedata.com/resource/pubmed/chemical/Lidocaine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Ptk2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, N-Methyl-D-Aspartate,
http://linkedlifedata.com/resource/pubmed/chemical/Ryanodine Receptor Calcium Release...,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrodotoxin,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0014-2999
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
489
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
55-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15063155-Anesthetics, Local,
pubmed-meshheading:15063155-Animals,
pubmed-meshheading:15063155-Blotting, Western,
pubmed-meshheading:15063155-Enzyme Inhibitors,
pubmed-meshheading:15063155-Focal Adhesion Kinase 1,
pubmed-meshheading:15063155-Focal Adhesion Protein-Tyrosine Kinases,
pubmed-meshheading:15063155-Hippocampus,
pubmed-meshheading:15063155-Lidocaine,
pubmed-meshheading:15063155-Male,
pubmed-meshheading:15063155-Phosphorylation,
pubmed-meshheading:15063155-Protein Kinase C,
pubmed-meshheading:15063155-Protein-Tyrosine Kinases,
pubmed-meshheading:15063155-Rats,
pubmed-meshheading:15063155-Rats, Sprague-Dawley,
pubmed-meshheading:15063155-Receptors, N-Methyl-D-Aspartate,
pubmed-meshheading:15063155-Ryanodine Receptor Calcium Release Channel,
pubmed-meshheading:15063155-Stimulation, Chemical,
pubmed-meshheading:15063155-Tetrodotoxin,
pubmed-meshheading:15063155-Tyrosine
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pubmed:year |
2004
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pubmed:articleTitle |
Lidocaine increases phosphorylation of focal adhesion kinase in rat hippocampal slices.
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pubmed:affiliation |
Institut National de la Santé et de la Recherche Médicale (INSERM) E 9935, Hôpital Robert Debré, 40 Bd Sérurier, 75019 Paris, France.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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