Source:http://linkedlifedata.com/resource/pubmed/id/15059848
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2004-7-2
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pubmed:abstractText |
CD8(+) T cells play a crucial role in the control of viral infections by direct elimination of infected cells and secretion of a number of soluble factors. Recent data suggest that HIV-1-specific CD8(+) T cell subsets may differ in their ability to exert these effector functions. Here, we directly compared the cytokine secretion patterns and cytotoxic capacity of HIV-1-specific CD8(+) T cells, using a flow-cytometric cytotoxicity assay based on caspase-3 activation in dying target cells. These experiments revealed considerable intraindividual and interindividual differences among epitope-specific T-cell effector functions: while the frequency of HIV-1-specific CD8(+) T cells secreting interferon-gamma but no tumor necrosis factor-alpha (TNF-alpha) following antigenic stimulation was only weakly correlated to their cytotoxic activity (R = 0.05, P =.57), a subset of CD8(+) T cells secreting both inter-feron-gamma and TNF-alpha was substantially more strongly associated with cytotoxicity (R = 0.67, P <.001). This subset of CD8(+) T cells also exhibited stronger intracellular perforin expression and more pronounced direct ex vivo HIV-1-specific cytoxicity than CD8(+) T cells secreting solely interferon-gamma following sorting of these subpopulations according to their cytokine profile. These results suggest that HIV-1-specific cytotoxicity of CD8(+) T cells is preferentially mediated by a subset of CD8(+) T cells secreting both interferon-gamma and TNF-alpha.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-4971
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pubmed:author |
pubmed-author:AddoMarylyn MMM,
pubmed-author:AllenTodd MTM,
pubmed-author:AlterGalitG,
pubmed-author:AltfeldMarcusM,
pubmed-author:CohenDanielD,
pubmed-author:JohnstonMary NMN,
pubmed-author:LichterfeldMathiasM,
pubmed-author:MuiStanley KSK,
pubmed-author:PaeEuniceE,
pubmed-author:RosenbergEric SES,
pubmed-author:StrickDaryldD,
pubmed-author:WalkerBruce DBD,
pubmed-author:WaringMichael TMT,
pubmed-author:YuXu GXG,
pubmed-author:ZaundersJohnJ
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
104
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
487-94
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15059848-Adult,
pubmed-meshheading:15059848-CD8-Positive T-Lymphocytes,
pubmed-meshheading:15059848-Caspase 3,
pubmed-meshheading:15059848-Caspases,
pubmed-meshheading:15059848-Female,
pubmed-meshheading:15059848-HIV Infections,
pubmed-meshheading:15059848-HIV-1,
pubmed-meshheading:15059848-Humans,
pubmed-meshheading:15059848-Immunophenotyping,
pubmed-meshheading:15059848-Interferon-gamma,
pubmed-meshheading:15059848-Male,
pubmed-meshheading:15059848-Middle Aged,
pubmed-meshheading:15059848-Substrate Specificity,
pubmed-meshheading:15059848-Tumor Necrosis Factor-alpha
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pubmed:year |
2004
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pubmed:articleTitle |
HIV-1-specific cytotoxicity is preferentially mediated by a subset of CD8(+) T cells producing both interferon-gamma and tumor necrosis factor-alpha.
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pubmed:affiliation |
Partners AIDS Research Center, Massachusetts General Hospital, 149 13th St, Rm 6613, Boston, MA 02129, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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