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rdf:type |
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lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0062565,
umls-concept:C0109317,
umls-concept:C0205263,
umls-concept:C0600432,
umls-concept:C0752312,
umls-concept:C0906802,
umls-concept:C1150579,
umls-concept:C1333340,
umls-concept:C1334474,
umls-concept:C1366882,
umls-concept:C1370600,
umls-concept:C1511938,
umls-concept:C1516960,
umls-concept:C1705767,
umls-concept:C1705791,
umls-concept:C1879547
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pubmed:issue |
1-2
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pubmed:dateCreated |
2004-3-19
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pubmed:abstractText |
K562 cells contain a Bcr-Abl chimeric gene and differentiate into various lineages in response to different inducers. We studied the role of the mitogen-activated protein kinase (MAPK) kinase 1 (MEK1)/extracellular signal-regulated kinase (ERK) pathway during the erythroid differentiation of K562 cells induced by tyrosine kinase inhibitors (herbimycin A or STI571), using genetically modified cells (constitutively MEK1-activated K562: K562/MEK1, and inducible ERK-inactivated K562: K562/CL100). Basal expression of glycophorin A was markedly reduced in K562/MEK1 cells compared with that in parental cells, while it was augmented in K562/CL100 cells. Herbimycin A and STI571 differentiated K562 cells accompanying with the transient down-regulated ERK. Moreover, the erythroid differentiation was markedly suppressed in K562/MEK1 cells, and early down-regulation of ERK activity was not observed in these cells. In contrast, the induction of ERK-specific phosphatase in K562/CL100 cells potentiated erythroid differentiation. Once the phosphatase was induced, the initial ERK activity became repressed and its early down-regulation by the inhibition of Bcr-Abl was marked and prolonged. These results demonstrate that the erythroid differentiation of K562 cells induced by herbimycin A or STI571 requires the down-regulation of MEK1/ ERK pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/B59 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Benzoquinones,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Fusion Proteins, bcr-abl,
http://linkedlifedata.com/resource/pubmed/chemical/Glycophorin,
http://linkedlifedata.com/resource/pubmed/chemical/Lactams, Macrocyclic,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/MAP2K1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Piperazines,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrimidines,
http://linkedlifedata.com/resource/pubmed/chemical/Quinones,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/herbimycin,
http://linkedlifedata.com/resource/pubmed/chemical/imatinib
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0300-8177
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
258
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
25-33
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15030167-Benzoquinones,
pubmed-meshheading:15030167-Cell Differentiation,
pubmed-meshheading:15030167-Enzyme Activation,
pubmed-meshheading:15030167-Enzyme Inhibitors,
pubmed-meshheading:15030167-Erythroblasts,
pubmed-meshheading:15030167-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:15030167-Fusion Proteins, bcr-abl,
pubmed-meshheading:15030167-Glycophorin,
pubmed-meshheading:15030167-Humans,
pubmed-meshheading:15030167-K562 Cells,
pubmed-meshheading:15030167-Lactams, Macrocyclic,
pubmed-meshheading:15030167-MAP Kinase Kinase 1,
pubmed-meshheading:15030167-Piperazines,
pubmed-meshheading:15030167-Protein Tyrosine Phosphatases,
pubmed-meshheading:15030167-Pyrimidines,
pubmed-meshheading:15030167-Quinones,
pubmed-meshheading:15030167-Recombinant Proteins,
pubmed-meshheading:15030167-Signal Transduction,
pubmed-meshheading:15030167-Transfection
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pubmed:year |
2004
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pubmed:articleTitle |
Inactivation of ERK accelerates erythroid differentiation of K562 cells induced by herbimycin A and STI571 while activation of MEK1 interferes with it.
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pubmed:affiliation |
Department of Molecular Genetics, Institute of DNA Medicine, Jikei University School of Medicine, Minato-ku, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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