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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1992-9-11
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pubmed:databankReference |
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pubmed:abstractText |
We cloned and sequenced a DNA fragment from Mycobacterium tuberculosis for use in the identification of members of the M. tuberculosis complex. The DNA probe for culture confirmation had a sensitivity and a specificity of 100%. By using primers developed from this probe, the polymerase chain reaction detected 20 mycobacteria by ethidium bromide staining. This polymerase chain reaction system demonstrated 98% sensitivity and 100% specificity for detection of the M. tuberculosis complex in 200 sputum specimens.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1500529-1903132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1500529-2063549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1500529-2106779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1500529-2109022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1500529-2120615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1500529-2121782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1500529-2123884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1500529-2203812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1500529-2572798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1500529-2909778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1500529-3082926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1500529-3139997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1500529-3140457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1500529-3932460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1500529-94515
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2173-6
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1500529-Bacteriological Techniques,
pubmed-meshheading:1500529-Base Sequence,
pubmed-meshheading:1500529-DNA, Bacterial,
pubmed-meshheading:1500529-DNA Probes,
pubmed-meshheading:1500529-Evaluation Studies as Topic,
pubmed-meshheading:1500529-Humans,
pubmed-meshheading:1500529-Molecular Sequence Data,
pubmed-meshheading:1500529-Mycobacterium,
pubmed-meshheading:1500529-Mycobacterium tuberculosis,
pubmed-meshheading:1500529-Polymerase Chain Reaction,
pubmed-meshheading:1500529-Sensitivity and Specificity,
pubmed-meshheading:1500529-Sputum,
pubmed-meshheading:1500529-Tuberculosis, Pulmonary
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pubmed:year |
1992
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pubmed:articleTitle |
Characterization of a DNA probe for detection of Mycobacterium tuberculosis complex in clinical samples by polymerase chain reaction.
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pubmed:affiliation |
Department of Pathology, University of British Columbia, Vancouver, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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