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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0018787,
umls-concept:C0020792,
umls-concept:C0026809,
umls-concept:C0070876,
umls-concept:C0185117,
umls-concept:C0205474,
umls-concept:C0392673,
umls-concept:C0596988,
umls-concept:C0872252,
umls-concept:C2911684
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pubmed:issue |
8
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pubmed:dateCreated |
2004-2-25
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pubmed:abstractText |
Phospholamban (PLN) is a critical regulator of cardiac contractility through its binding to and regulation of the activity of the sarco(endo)plasmic reticulum Ca2+ ATPase. To uncover biochemical adaptations associated with extremes of cardiac muscle contractility, we used high-throughput gel-free tandem MS to monitor differences in the relative abundance of membrane proteins in standard microsomal fractions isolated from the hearts of PLN-null mice (PLN-KO) with high contractility and from transgenic mice overexpressing a superinhibitory PLN mutant in a PLN-null background (I40A-KO) with diminished contractility. Significant differential expression was detected for a subset of the 782 proteins identified, including known membrane-associated biomarkers, components of signaling pathways, and previously uninvestigated proteins. Proteins involved in fat and carbohydrate metabolism and proteins linked to G protein-signaling pathways activating protein kinase C were enriched in I40A-KO cardiac muscle, whereas proteins linked to enhanced contractile function were enriched in PLN-KO mutant hearts. These data demonstrate that Ca2+ dysregulation, leading to elevated or depressed cardiac contractility, induces compensatory biochemical responses.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-10592178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-10728347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-10744747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-10786884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-10805514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-10809743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-10870955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-11181961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-11181995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-11231557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-11325867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-11328820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-11343424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-11752248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-12368866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-12466850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-12505056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-12634793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-12639993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-12644571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-12700003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-12818578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-12838339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-14575316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-1835945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-2122001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-2907916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-3759968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-8062415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-8943944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-9150937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-9223325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-9576430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-9710796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-9740046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14982994-9740065
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
101
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2241-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:14982994-Animals,
pubmed-meshheading:14982994-Atrial Natriuretic Factor,
pubmed-meshheading:14982994-Base Sequence,
pubmed-meshheading:14982994-Calcium-Binding Proteins,
pubmed-meshheading:14982994-Enzymes,
pubmed-meshheading:14982994-Gene Expression Profiling,
pubmed-meshheading:14982994-Genotype,
pubmed-meshheading:14982994-Mice,
pubmed-meshheading:14982994-Mice, Knockout,
pubmed-meshheading:14982994-Mice, Transgenic,
pubmed-meshheading:14982994-Microsomes,
pubmed-meshheading:14982994-Molecular Sequence Data,
pubmed-meshheading:14982994-Myocardial Contraction,
pubmed-meshheading:14982994-Myosin Heavy Chains,
pubmed-meshheading:14982994-Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
Identification of biochemical adaptations in hyper- or hypocontractile hearts from phospholamban mutant mice by expression proteomics.
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pubmed:affiliation |
Banting and Best Department of Medical Research, University of Toronto, Toronto, ON, Canada M5G 1L6.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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