Linked Life Data

1478960

Source:http://linkedlifedata.com/resource/pubmed/id/1478960

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1993-2-8
pubmed:abstractText
Dynamin, a microtubule-activated GTPase, has recently been identified as the product of the shibire gene in Drosophila. shi(ts) mutants are defective in synaptic vesicle recycling, which leads to rapid and reversible temperature-sensitive paralysis. In the present study, results from RNase protection assays and analysis of cDNA clones define a complex pattern of developmental- and tissue-specific alternative splicing at two sites within the coding region. This analysis is also supported by western blot analysis with two polyclonal antibodies. In situ hybridization data revealed a high concentration of shi transcripts in the central and peripheral nervous system throughout neuronal development. Other than the nervous system, shi transcripts are also expressed at a high level in early embryos, larval imaginal discs, and male and female gonads. These data provide a basis for interpreting the wide range of phenotypic effects of shi mutations in terms of the putative membrane-sorting properties of dynamin and for further functional study of different dynamin isoforms.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:volume
103 ( Pt 3)
pubmed:geneSymbol
shi
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
619-28
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Developmental stage- and tissue-specific expression of shibire, a Drosophila gene involved in endocytosis.
pubmed:affiliation
Cell Biology Group, Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.