Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-2-2
pubmed:abstractText
Signal transduction pathways are modular composites of functionally interdependent sets of proteins that act in a coordinated fashion to transform environmental information into a phenotypic response. The pro-inflammatory cytokine tumour necrosis factor (TNF)-alpha triggers a signalling cascade, converging on the activation of the transcription factor NF-kappa B, which forms the basis for numerous physiological and pathological processes. Here we report the mapping of a protein interaction network around 32 known and candidate TNF-alpha/NF-kappa B pathway components by using an integrated approach comprising tandem affinity purification, liquid-chromatography tandem mass spectrometry, network analysis and directed functional perturbation studies using RNA interference. We identified 221 molecular associations and 80 previously unknown interactors, including 10 new functional modulators of the pathway. This systems approach provides significant insight into the logic of the TNF-alpha/NF-kappa B pathway and is generally applicable to other pathways relevant to human disease.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/CDC37 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Chaperonins, http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins, http://linkedlifedata.com/resource/pubmed/chemical/HSP90 Heat-Shock Proteins, http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins, http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase Kinase 3, http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase Kinases, http://linkedlifedata.com/resource/pubmed/chemical/MAP3K3 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/MCC protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances, http://linkedlifedata.com/resource/pubmed/chemical/Molecular Chaperones, http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B, http://linkedlifedata.com/resource/pubmed/chemical/Proteome, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor, http://linkedlifedata.com/resource/pubmed/chemical/Tacrolimus Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha, http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins, http://linkedlifedata.com/resource/pubmed/chemical/tacrolimus binding protein 5
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1465-7392
pubmed:author
pubmed-author:AngrandPierre-OlivierPO, pubmed-author:BauchAngelaA, pubmed-author:BauerAndreasA, pubmed-author:BergaminiGiovannaG, pubmed-author:BouwmeesterTewisT, pubmed-author:CasariGeorgG, pubmed-author:CroughtonKarenK, pubmed-author:CruciatCristinaC, pubmed-author:DrewesGerardG, pubmed-author:EberhardDirkD, pubmed-author:GagneurJulienJ, pubmed-author:GavinAnne-ClaudeAC, pubmed-author:GhidelliSonjaS, pubmed-author:HopfCarstenC, pubmed-author:HuhseBettinaB, pubmed-author:JacksonDavid BDB, pubmed-author:JobertyGerardG, pubmed-author:KusterBernhardB, pubmed-author:ManganoRaffaellaR, pubmed-author:MichonAnne-MarieAM, pubmed-author:NeubauerGitteG, pubmed-author:RickJensJ, pubmed-author:RuffnerHeinzH, pubmed-author:SchirleMarkusM, pubmed-author:SchleglJudithJ, pubmed-author:SchwabMarkusM, pubmed-author:SteinMartin AMA, pubmed-author:Superti-FurgaGiulioG
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
97-105
pubmed:dateRevised
2011-11-2
pubmed:meshHeading
pubmed-meshheading:14743216-Animals, pubmed-meshheading:14743216-Carrier Proteins, pubmed-meshheading:14743216-Cell Cycle Proteins, pubmed-meshheading:14743216-Cell Line, pubmed-meshheading:14743216-Chaperonins, pubmed-meshheading:14743216-Chromatography, Affinity, pubmed-meshheading:14743216-Drosophila Proteins, pubmed-meshheading:14743216-Enzyme Activation, pubmed-meshheading:14743216-HSP90 Heat-Shock Proteins, pubmed-meshheading:14743216-Humans, pubmed-meshheading:14743216-I-kappa B Proteins, pubmed-meshheading:14743216-MAP Kinase Kinase Kinase 3, pubmed-meshheading:14743216-MAP Kinase Kinase Kinases, pubmed-meshheading:14743216-Macromolecular Substances, pubmed-meshheading:14743216-Mass Spectrometry, pubmed-meshheading:14743216-Models, Biological, pubmed-meshheading:14743216-Molecular Chaperones, pubmed-meshheading:14743216-NF-kappa B, pubmed-meshheading:14743216-Proteome, pubmed-meshheading:14743216-RNA Interference, pubmed-meshheading:14743216-Receptors, Tumor Necrosis Factor, pubmed-meshheading:14743216-Signal Transduction, pubmed-meshheading:14743216-Tacrolimus Binding Proteins, pubmed-meshheading:14743216-Tumor Necrosis Factor-alpha, pubmed-meshheading:14743216-Tumor Suppressor Proteins
pubmed:year
2004
pubmed:articleTitle
A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway.
pubmed:affiliation
Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany. tewis.bouwmeester@cellzome.com
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