Linked Life Data

14722224

Source:http://linkedlifedata.com/resource/pubmed/id/14722224

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2004-5-24
pubmed:abstractText
Respiratory viruses often express mechanisms to resist host antiviral systems, but the biochemical basis for evasion of interferon effects by respiratory syncytial virus (RSV) is poorly defined. In this study, we identified RSV effects on interferon (IFN)-dependent signal transduction and gene expression in human airway epithelial cells. Initial experiments demonstrated inhibition of antiviral gene expression induced by IFN-alpha and IFN-beta, but not IFN-gamma, in epithelial cells infected with RSV. Selective viral effects on type I IFN-dependent signaling were confirmed when we observed impaired type I, but not type II, IFN-induced activation of the transcription factor Stat1 in RSV-infected cells. RSV infection of airway epithelial cells resulted in decreased Stat2 expression and function with preservation of upstream signaling events, providing a molecular mechanism for viral inhibition of the type I IFN JAK-STAT pathway. Furthermore, nonspecific pharmacologic inhibition of proteasome function in RSV-infected cells restored Stat2 levels and IFN-dependent activation of Stat1. The results indicate that RSV acts on epithelial cells in the airway to directly modulate the type I IFN JAK-STAT pathway, and this effect is likely mediated though proteasome-dependent degradation of Stat2. Decreased antiviral gene expression in RSV-infected airway epithelial cells may allow RSV replication and establishment of a productive viral infection through subversion of IFN-dependent immunity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1044-1549
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
893-900
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14722224-Animals, pubmed-meshheading:14722224-Cells, Cultured, pubmed-meshheading:14722224-Cysteine Endopeptidases, pubmed-meshheading:14722224-DNA-Binding Proteins, pubmed-meshheading:14722224-Epithelial Cells, pubmed-meshheading:14722224-Gene Expression Regulation, pubmed-meshheading:14722224-Humans, pubmed-meshheading:14722224-Interferon Type I, pubmed-meshheading:14722224-Multienzyme Complexes, pubmed-meshheading:14722224-Proteasome Endopeptidase Complex, pubmed-meshheading:14722224-Respiratory Mucosa, pubmed-meshheading:14722224-Respiratory Syncytial Virus Infections, pubmed-meshheading:14722224-Respiratory Syncytial Viruses, pubmed-meshheading:14722224-STAT1 Transcription Factor, pubmed-meshheading:14722224-STAT2 Transcription Factor, pubmed-meshheading:14722224-Signal Transduction, pubmed-meshheading:14722224-Trans-Activators
pubmed:year
2004
pubmed:articleTitle
Specific inhibition of type I interferon signal transduction by respiratory syncytial virus.
pubmed:affiliation
University of Iowa Roy J. and Lucille A. Carver College of Medicine, Department of Internal Medicine, 200 Hawkins Drive, C33-GH, Iowa City, IA 52242, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't