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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2004-1-13
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pubmed:abstractText |
During erythroid differentiation, beta-globin gene expression is regulated by the locus control region (LCR). The transcription factor NF-E2p18/MafK binds within this region and is essential for beta-globin expression in murine erythroleukemia (MEL) cells. Here we use the isotope-coded affinity tag (ICAT) technique of quantitative mass spectrometry to compare proteins interacting with NF-E2p18/MafK during differentiation. Our results define MafK as a 'dual-function' molecule that shifts from a repressive to an activating mode during erythroid differentiation. The exchange of MafK dimerization partner from Bach1 to NF-E2p45 is a key step in the switch from the repressed to the active state. This shift is associated with changes in the interaction of MafK with co-repressors and co-activators. Thus, our results suggest that in addition to its role as a cis-acting activator of beta-globin gene expression in differentiated erythroid cells, the LCR also promotes an active repression of beta-globin transcription in committed cells before terminal differentiation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1545-9993
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
73-80
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:14718926-Animals,
pubmed-meshheading:14718926-Cell Differentiation,
pubmed-meshheading:14718926-Cell Line,
pubmed-meshheading:14718926-DNA-Binding Proteins,
pubmed-meshheading:14718926-Erythrocytes,
pubmed-meshheading:14718926-Erythroid-Specific DNA-Binding Factors,
pubmed-meshheading:14718926-Erythropoiesis,
pubmed-meshheading:14718926-Gene Expression Regulation, Developmental,
pubmed-meshheading:14718926-Globins,
pubmed-meshheading:14718926-Macromolecular Substances,
pubmed-meshheading:14718926-MafK Transcription Factor,
pubmed-meshheading:14718926-Mice,
pubmed-meshheading:14718926-Models, Biological,
pubmed-meshheading:14718926-Nuclear Proteins,
pubmed-meshheading:14718926-Proteomics,
pubmed-meshheading:14718926-Transcription Factors
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pubmed:year |
2004
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pubmed:articleTitle |
Dynamic changes in transcription factor complexes during erythroid differentiation revealed by quantitative proteomics.
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pubmed:affiliation |
Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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