Source:http://linkedlifedata.com/resource/pubmed/id/14715266
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2004-1-12
|
| pubmed:abstractText |
Signaling pathways involved in oxidative stress-induced inhibition of osteoblast differentiation are not known. We showed in this report that H(2)O(2) (0.1-0.2mM)-induced oxidative stress suppressed the osteoblastic differentiation process of primary rabbit bone marrow stromal cells (BMSC) and calvarial osteoblasts, manifested by a reduction of differentiation markers including alkaline phosphatase (ALP), type I collagen, colony-forming unit-osteoprogenitor (CFU-O) formation, and nuclear phosphorylation of Runx2. H(2)O(2) treatment stimulated phospholipase C-gamma1 (PLC-gamma1), extracellular signal-regulated kinase 1/2 (ERK1/2), and NF-kappaB signaling but inhibited p38 mitogen-activated protein kinase (MAPK) activation. In the presence of 20microM PD98059 or 50microM caffeic acid phenethyl ester (CAPE), specific inhibitor for ERKs or NF-kappaB, respectively, could significantly reverse the decrease of above-mentioned osteoblastic differentiation markers elicited by H(2)O(2) (0.1mM). Furthermore, PD98059 also suppressed H(2)O(2)-stimulated NF-kappaB signaling in this process. These data suggest that ERK and ERK-dependent NF-kappaB activation is required for oxidative stress-induced inhibition of osteoblastic differentiation in rabbit BMSC and calvarial osteoblasts.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
30
|
| pubmed:volume |
314
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
197-207
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:14715266-Animals,
pubmed-meshheading:14715266-Bone Marrow Cells,
pubmed-meshheading:14715266-Cell Differentiation,
pubmed-meshheading:14715266-Cell Line,
pubmed-meshheading:14715266-Cell Survival,
pubmed-meshheading:14715266-Dose-Response Relationship, Drug,
pubmed-meshheading:14715266-Hydrogen Peroxide,
pubmed-meshheading:14715266-Mitogen-Activated Protein Kinases,
pubmed-meshheading:14715266-NF-kappa B,
pubmed-meshheading:14715266-Osteoblasts,
pubmed-meshheading:14715266-Oxidative Stress,
pubmed-meshheading:14715266-Rabbits,
pubmed-meshheading:14715266-Signal Transduction,
pubmed-meshheading:14715266-Skull
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Oxidative stress inhibits osteoblastic differentiation of bone cells by ERK and NF-kappaB.
|
| pubmed:affiliation |
Department of Cell Biology, The First Military Medical University, Guangzhou, PR China.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|