| pubmed-article:14685298 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14685298 | lifeskim:mentions | umls-concept:C0040300 | lld:lifeskim |
| pubmed-article:14685298 | lifeskim:mentions | umls-concept:C0597032 | lld:lifeskim |
| pubmed-article:14685298 | lifeskim:mentions | umls-concept:C0431085 | lld:lifeskim |
| pubmed-article:14685298 | lifeskim:mentions | umls-concept:C0005220 | lld:lifeskim |
| pubmed-article:14685298 | lifeskim:mentions | umls-concept:C0242485 | lld:lifeskim |
| pubmed-article:14685298 | lifeskim:mentions | umls-concept:C0441889 | lld:lifeskim |
| pubmed-article:14685298 | lifeskim:mentions | umls-concept:C1520166 | lld:lifeskim |
| pubmed-article:14685298 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:14685298 | pubmed:dateCreated | 2003-12-19 | lld:pubmed |
| pubmed-article:14685298 | pubmed:abstractText | The nude mouse xenograft model is commonly used to examine the growth and development of human cancer cells in vivo. Tumor cells transfected with the Lac-Z reporter gene for beta-galactosidase (beta-gal) enzyme activity can be used to quantify tumor and metastatic development in this model. The present study was designed to develop methodology to accurately measure beta-gal in tumor and tissue samples from a nude mouse model. In this study, we developed tissue extraction procedures and compared the sensitivity and accuracy of o-nitrophenyl-beta-D-galactopyranoside (ONPG) and chlorophenol red beta-D-galactopyranoside (CPRG); two beta-gal substrates. Our results demonstrated that the CPRG substrate is more sensitive and accurate in the measurement of beta-gal activity than the ONPG substrate. In addition, matrices and blood in tissue samples are less likely to interfere with the CPRG assay. We concluded that the CPRG substrate-based assay represents a reliable technique for the determination of beta-gal activity in transfected cancer cells present in tumor and tissue specimens from the nude mouse xenograft model. | lld:pubmed |
| pubmed-article:14685298 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14685298 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14685298 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14685298 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14685298 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14685298 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14685298 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:14685298 | pubmed:issn | 0379-0355 | lld:pubmed |
| pubmed-article:14685298 | pubmed:author | pubmed-author:HowardE WEW | lld:pubmed |
| pubmed-article:14685298 | pubmed:author | pubmed-author:PentoJ TJT | lld:pubmed |
| pubmed-article:14685298 | pubmed:author | pubmed-author:BullerC JCJ | lld:pubmed |
| pubmed-article:14685298 | pubmed:author | pubmed-author:ZangX-PXP | lld:pubmed |
| pubmed-article:14685298 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14685298 | pubmed:volume | 25 | lld:pubmed |
| pubmed-article:14685298 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14685298 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14685298 | pubmed:pagination | 713-6 | lld:pubmed |
| pubmed-article:14685298 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:14685298 | pubmed:meshHeading | pubmed-meshheading:14685298... | lld:pubmed |
| pubmed-article:14685298 | pubmed:meshHeading | pubmed-meshheading:14685298... | lld:pubmed |
| pubmed-article:14685298 | pubmed:meshHeading | pubmed-meshheading:14685298... | lld:pubmed |
| pubmed-article:14685298 | pubmed:meshHeading | pubmed-meshheading:14685298... | lld:pubmed |
| pubmed-article:14685298 | pubmed:meshHeading | pubmed-meshheading:14685298... | lld:pubmed |
| pubmed-article:14685298 | pubmed:meshHeading | pubmed-meshheading:14685298... | lld:pubmed |
| pubmed-article:14685298 | pubmed:meshHeading | pubmed-meshheading:14685298... | lld:pubmed |
| pubmed-article:14685298 | pubmed:meshHeading | pubmed-meshheading:14685298... | lld:pubmed |
| pubmed-article:14685298 | pubmed:meshHeading | pubmed-meshheading:14685298... | lld:pubmed |
| pubmed-article:14685298 | pubmed:meshHeading | pubmed-meshheading:14685298... | lld:pubmed |
| pubmed-article:14685298 | pubmed:meshHeading | pubmed-meshheading:14685298... | lld:pubmed |
| pubmed-article:14685298 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:14685298 | pubmed:articleTitle | Measurement of beta-galactosidase tissue levels in a tumor cell xenograft model. | lld:pubmed |
| pubmed-article:14685298 | pubmed:affiliation | Department of Pharmaceutical Sciences, University of Oklahoma Health Sciences Center, Oklahoma City, USA. | lld:pubmed |
| pubmed-article:14685298 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14685298 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:14685298 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:14685298 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14685298 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14685298 | lld:pubmed |
