Linked Life Data

14685298

Source:http://linkedlifedata.com/resource/pubmed/id/14685298

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2003-12-19
pubmed:abstractText
The nude mouse xenograft model is commonly used to examine the growth and development of human cancer cells in vivo. Tumor cells transfected with the Lac-Z reporter gene for beta-galactosidase (beta-gal) enzyme activity can be used to quantify tumor and metastatic development in this model. The present study was designed to develop methodology to accurately measure beta-gal in tumor and tissue samples from a nude mouse model. In this study, we developed tissue extraction procedures and compared the sensitivity and accuracy of o-nitrophenyl-beta-D-galactopyranoside (ONPG) and chlorophenol red beta-D-galactopyranoside (CPRG); two beta-gal substrates. Our results demonstrated that the CPRG substrate is more sensitive and accurate in the measurement of beta-gal activity than the ONPG substrate. In addition, matrices and blood in tissue samples are less likely to interfere with the CPRG assay. We concluded that the CPRG substrate-based assay represents a reliable technique for the determination of beta-gal activity in transfected cancer cells present in tumor and tissue specimens from the nude mouse xenograft model.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0379-0355
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
713-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Measurement of beta-galactosidase tissue levels in a tumor cell xenograft model.
pubmed:affiliation
Department of Pharmaceutical Sciences, University of Oklahoma Health Sciences Center, Oklahoma City, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't